Run-on transcription as a method for the analysis of mitochondrial genome expression

Run-on transcription as a method for the analysis of mitochondrial genome expression The main stages of the run-on transcription method as applied to the quantification of mitochondrial gene transcription are described. As distinct from widely applied methods of northern hybridization and PCR after reverse transcription, this method permits studying the effects of exogenous and endogenous factors on the rate of mitochondrial gene transcription. The main method stages are (1) isolation of intact organelles; (2) preparation of nylon membranes with gene-specific fragments of DNA; (3) transcription in the lysates of these organelles in the presence of 32P-labeled RNA precursors; and (4) hybridization of newly synthesized labeled transcripts with DNA fragments of tested mitochondrial genes loaded on the nylon membrane. Russian Journal of Plant Physiology Springer Journals

Run-on transcription as a method for the analysis of mitochondrial genome expression

Loading next page...
SP MAIK Nauka/Interperiodica
Copyright © 2011 by Pleiades Publishing, Ltd.
Life Sciences; Plant Sciences ; Plant Physiology
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial