The authors describe an antibody-aptamer based hetero-sandwich amperometric biosensor for the foodborne pathogen Vibrio parahaemolyticus. Antibody on the surface of a gold electrode first captures the target pathogen, and then the immunocomplex is incubated with an aptamer consisting of an ssDNA probe. A hetero-sandwich structure is formed which allows for signal enhancement by rolling circle amplification. Following addition of Methylene Blue as an electrochemical DNA probe, the amperometric signal, best measured at 0.28 V (vs. Ag/AgCl), covers the 2.2 to 2.2*108 cfu per mL concentration range, and the limit of detection is as low as 2 cfu per mL. The method was successfully applied to the quantification of V. parahaemolyticus in spiked fish samples. In our perception, this hetero-sandwich electrochemical biosensor provides an ultrasensitive tool for the quantitation of a variety of pathogen if appropriate antibodies and aptamers are available.
Microchimica Acta – Springer Journals
Published: Jun 22, 2017
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