Rolling circle amplification based amperometric aptamer/immuno hybrid biosensor for ultrasensitive detection of Vibrio parahaemolyticus

Rolling circle amplification based amperometric aptamer/immuno hybrid biosensor for... The authors describe an antibody-aptamer based hetero-sandwich amperometric biosensor for the foodborne pathogen Vibrio parahaemolyticus. Antibody on the surface of a gold electrode first captures the target pathogen, and then the immunocomplex is incubated with an aptamer consisting of an ssDNA probe. A hetero-sandwich structure is formed which allows for signal enhancement by rolling circle amplification. Following addition of Methylene Blue as an electrochemical DNA probe, the amperometric signal, best measured at 0.28 V (vs. Ag/AgCl), covers the 2.2 to 2.2*108 cfu per mL concentration range, and the limit of detection is as low as 2 cfu per mL. The method was successfully applied to the quantification of V. parahaemolyticus in spiked fish samples. In our perception, this hetero-sandwich electrochemical biosensor provides an ultrasensitive tool for the quantitation of a variety of pathogen if appropriate antibodies and aptamers are available. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Microchimica Acta Springer Journals

Rolling circle amplification based amperometric aptamer/immuno hybrid biosensor for ultrasensitive detection of Vibrio parahaemolyticus

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Publisher
Springer Vienna
Copyright
Copyright © 2017 by Springer-Verlag GmbH Austria
Subject
Chemistry; Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering
ISSN
0026-3672
eISSN
1436-5073
D.O.I.
10.1007/s00604-017-2383-0
Publisher site
See Article on Publisher Site

Abstract

The authors describe an antibody-aptamer based hetero-sandwich amperometric biosensor for the foodborne pathogen Vibrio parahaemolyticus. Antibody on the surface of a gold electrode first captures the target pathogen, and then the immunocomplex is incubated with an aptamer consisting of an ssDNA probe. A hetero-sandwich structure is formed which allows for signal enhancement by rolling circle amplification. Following addition of Methylene Blue as an electrochemical DNA probe, the amperometric signal, best measured at 0.28 V (vs. Ag/AgCl), covers the 2.2 to 2.2*108 cfu per mL concentration range, and the limit of detection is as low as 2 cfu per mL. The method was successfully applied to the quantification of V. parahaemolyticus in spiked fish samples. In our perception, this hetero-sandwich electrochemical biosensor provides an ultrasensitive tool for the quantitation of a variety of pathogen if appropriate antibodies and aptamers are available.

Journal

Microchimica ActaSpringer Journals

Published: Jun 22, 2017

References

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