Access the full text.
Sign up today, get DeepDyve free for 14 days.
T. Baukrowitz, G. Yellen (1996)
Use-Dependent Blockers and Exit Rate of the Last Ion from the Multi-Ion Pore of a K+ ChannelScience, 271
E. Ogielska, W. Zagotta, Toshinori Hoshi, S. Heinemann, J. Haab, R. Aldrich (1995)
Cooperative subunit interactions in C-type inactivation of K channels.Biophysical journal, 69 6
Ba, Mg, Na, N. Khan, I. Gray, C. Obejero-Paz, S. Jones (2008)
Permeation and Gating in CaV3.1 (α1G) T-type Calcium Channels Effects of Ca2+, Ba2+, Mg2+, and Na+The Journal of General Physiology, 132
Kevin Dougherty, J. Santiago-Castillo, M. Covarrubias (2008)
Gating Charge Immobilization in Kv4.2 Channels: The Basis of Closed-State InactivationThe Journal of General Physiology, 131
R. Aldrich, P. Getting, S. Thompson (1979)
Mechanism of frequency‐dependent broadening of molluscan neurone soma spikes.The Journal of Physiology, 291
R. Rasmusson, M. Morales, R. Castellino, Y. Zhang, D. Campbell, H. Strauss (1995)
C‐type inactivation controls recovery in a fast inactivating cardiac K+ channel (Kv1.4) expressed in Xenopus oocytes.The Journal of Physiology, 489
J. Cordero-Morales, J. Cordero-Morales, V. Jogini, A. Lewis, V. Vásquez, V. Vásquez, D. Cortes, B. Roux, E. Perozo (2007)
Molecular driving forces determining potassium channel slow inactivationNature Structural &Molecular Biology, 14
J. Cordero-Morales, J. Cordero-Morales, L. Cuello, L. Cuello, Yanxiang Zhao, V. Jogini, V. Jogini, D. Cortes, D. Cortes, Benoît Roux, Benoît Roux, E. Perozo, E. Perozo (2006)
Molecular determinants of gating at the potassium-channel selectivity filterNature Structural &Molecular Biology, 13
Harley Kurata, Kyle Doerksen, J. Eldstrom, Saman Rezazadeh, D. Fedida (2005)
Separation of P/C‐ and U‐type inactivation pathways in Kv1.5 potassium channelsThe Journal of Physiology, 568
Harley Kurata, Gordon Soon, J. Eldstrom, Grace Lu, D. Steele, D. Fedida (2002)
Amino-terminal Determinants of U-type Inactivation of Voltage-gated K+ Channels*The Journal of Biological Chemistry, 277
D. Fedida, Neil Maruoka, Shunping Lin (1999)
Modulation of slow inactivation in human cardiac Kv1.5 channels by extra‐ and intracellular permeant cationsThe Journal of Physiology, 515
A. Hodgkin, A. Huxley (1952)
Currents carried by sodium and potassium ions through the membrane of the giant axon of LoligoThe Journal of Physiology, 116
Harley Kurata, Gordon Soon, D. Fedida (2001)
Altered State Dependence of C-Type Inactivation in the Long and Short Forms of Human Kv1.5The Journal of General Physiology, 118
J. López-Barneo, T. Hoshi, S. Heinemann, R. Aldrich (1993)
Effects of external cations and mutations in the pore region on C-type inactivation of Shaker potassium channels.Receptors & channels, 1 1
L. Cuello, V. Jogini, D. Cortes, E. Perozo (2010)
Structural mechanism of C-type inactivation in K+ channelsNature, 466
C. Frazier, E. George, S. Jones (2000)
Apparent change in ion selectivity caused by changes in intracellular K(+) during whole-cell recording.Biophysical journal, 78 4
D. Jacobson, A. Kuznetsov, James Lopez, S. Kash, C. Ammälä, L. Philipson (2007)
Kv2.1 ablation alters glucose-induced islet electrical activity, enhancing insulin secretion.Cell metabolism, 6 3
A. Nguyen, J. Kath, D. Hanson, M. Biggers, P. Canniff, C. Donovan, R. Mather, Matthew Bruns, H. Rauer, J. Aiyar, A. Lepple-Wienhues, G. Gutman, S. Grissmer, M. Cahalan, K. Chandy (1996)
Novel nonpeptide agents potently block the C-type inactivated conformation of Kv1.3 and suppress T cell activation.Molecular pharmacology, 50 6
Roderick MacKinnon, G. Yellen (1990)
Mutations affecting TEA blockade and ion permeation in voltage-activated K+ channels.Science, 250 4978
Paula Smith, T. Baukrowitz, G. Yellen (1996)
The inward rectification mechanism of the HERG cardiac potassium channelNature, 379
D. Colquhoun, A. Hawkes (1995)
A Q-Matrix Cookbook
Yi Liu, M. Jurman, G. Yellen (1996)
Dynamic Rearrangement of the Outer Mouth of a K+ Channel during GatingNeuron, 16
T. Hoshi, W. Zagotta, R. Aldrich (1990)
Biophysical and molecular mechanisms of Shaker potassium channel inactivationScience, 250
Jan Barghaan, R. Bähring (2009)
Dynamic Coupling of Voltage Sensor and Gate Involved in Closed-State Inactivation of Kv4.2 ChannelsThe Journal of General Physiology, 133
K. Klemic, G. Kirsch, G. Kirsch, S. Jones (2001)
U-type inactivation of Kv3.1 and Shaker potassium channels.Biophysical journal, 81 2
Jones: Outer-pore Residue Y380
(1995)
A Q-matric cookbook How to write only one program to calculate the single-channel and macroscopic predictions for any kinetic mechanism
D. Kerschensteiner, Francisco Monje, M. Stocker (2003)
Structural Determinants of the Regulation of the Voltage-gated Potassium Channel Kv2.1 by the Modulatory α-Subunit Kv9.3*The Journal of Biological Chemistry, 278
S. Grissmer, M. Cahalan (1989)
TEA prevents inactivation while blocking open K+ channels in human T lymphocytes.Biophysical journal, 55 1
Kathleen Choi, R. Aldrich, G. Yellen, G. Yellen (1991)
Tetraethylammonium blockade distinguishes two inactivation mechanisms in voltage-activated K+ channels.Proceedings of the National Academy of Sciences of the United States of America, 88
R. Bähring, L. Boland, A. Varghese, Manuel Gebauer, O. Pongs (2001)
Kinetic analysis of open‐ and closed‐state inactivation transitions in human Kv4.2 A‐type potassium channelsThe Journal of Physiology, 535
Y. Cheng, J. Azer, C. Niven, P. Mafi, C. Allard, J. Qi, S. Thouta, T. Claydon (2011)
Molecular determinants of U-type inactivation in Kv2.1 channels.Biophysical journal, 101 3
G. Frech, A. VanDongen, G. Schuster, A. Brown, R. Joho (1989)
A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloningNature, 340
J. Connor, C. Stevens (1971)
Inward and delayed outward membrane currents in isolated neural somata under voltage clampThe Journal of Physiology, 213
B. Sakmann, E. Neher (1995)
Single-Channel Recording
H. Jerng, M. Shahidullah, M. Covarrubias (1999)
Inactivation Gating of Kv4 Potassium Channels Molecular Interactions Involving the Inner Vestibule of the Pore
T. Hoshi, W. Zagotta, R. Aldrich (1991)
Two types of inactivation in Shaker K+ channels: Effects of alterations in the carboxy-terminal regionNeuron, 7
K. Klemic, C. Shieh, C. Shieh, G. Kirsch, G. Kirsch, S. Jones (1998)
Inactivation of Kv2.1 potassium channels.Biophysical journal, 74 4
The interpretation of slow inactivation in potassium channels has been strongly influenced by work on C-type inactivation in Shaker channels. Slow inactivation in Shaker and some other potassium channels can be dramatically modulated by the state of the pore, including mutations at outer pore residue T449, which altered inactivation kinetics up to 100-fold. KV2.1, another voltage-dependent potassium channel, exhibits a biophysically distinct inactivation mechanism with a U-shaped voltage-dependence and preferential closed-state inactivation, termed U-type inactivation. However, it remains to be demonstrated whether U-type and C-type inactivation have different molecular mechanisms. This study examines mutations at Y380 (homologous to Shaker T449) to investigate whether C-type and U-type inactivation have distinct molecular mechanisms, and whether C-type inactivation can occur at all in KV2.1. Y380 mutants do not introduce C-type inactivation into KV2.1 and have little effect on U-type inactivation of KV2.1. Interestingly, two of the mutants tested exhibit twofold faster recovery from inactivation compared to wild-type channels. The observation that mutations have little effect suggests KV2.1 lacks C-type inactivation as it exists in Shaker and that C-type and U-type inactivation have different molecular mechanisms. Kinetic modeling predicts that all mutants inactivate preferentially, but not exclusively, from partially activated closed states. Therefore, KV2.1 exhibits a single U-type inactivation process including some inactivation from open as well as closed states.
The Journal of Membrane Biology – Springer Journals
Published: Jun 28, 2013
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.