Role of cell death in the propagation of PrP Sc in immune cells

Role of cell death in the propagation of PrP Sc in immune cells A number of studies have suggested that macrophages, dendritic cells, and follicular dendritic cells play an important role in the propagation of PrP Sc . Both accumulation and proteolysis of PrP Sc have been demonstrated in peripheral macrophages. Macrophages may act as reservoirs for PrP Sc particles if the cells die during transient PrP Sc propagation. However, whether cell death plays a role in PrP Sc propagation in macrophages remains unclear. In this study, we investigated the possibility of propagation and transmission of PrP Sc between dead immune cells and living neural cells. We found that under specific conditions, transient PrP Sc propagation occurs in dead cells, indicating that interaction between PrP C and PrP Sc on plasma membrane lipid rafts might be important for PrP Sc propagation. Co-culturing of killed donor PrP Sc -infected macrophages with recipient N2a-3 neuroblastoma cells accelerated PrP Sc transmission. Our results suggest that cell death may play an important role in PrP Sc propagation, whereas transient PrP Sc propagation in macrophages has little effect on PrP Sc transmission. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Role of cell death in the propagation of PrP Sc in immune cells

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Publisher
Springer Journals
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2320-z
Publisher site
See Article on Publisher Site

Abstract

A number of studies have suggested that macrophages, dendritic cells, and follicular dendritic cells play an important role in the propagation of PrP Sc . Both accumulation and proteolysis of PrP Sc have been demonstrated in peripheral macrophages. Macrophages may act as reservoirs for PrP Sc particles if the cells die during transient PrP Sc propagation. However, whether cell death plays a role in PrP Sc propagation in macrophages remains unclear. In this study, we investigated the possibility of propagation and transmission of PrP Sc between dead immune cells and living neural cells. We found that under specific conditions, transient PrP Sc propagation occurs in dead cells, indicating that interaction between PrP C and PrP Sc on plasma membrane lipid rafts might be important for PrP Sc propagation. Co-culturing of killed donor PrP Sc -infected macrophages with recipient N2a-3 neuroblastoma cells accelerated PrP Sc transmission. Our results suggest that cell death may play an important role in PrP Sc propagation, whereas transient PrP Sc propagation in macrophages has little effect on PrP Sc transmission.

Journal

Archives of VirologySpringer Journals

Published: Mar 1, 2015

References

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