RNA-seq transcriptional profiling of Herbaspirillum seropedicae colonizing wheat (Triticum aestivum) roots

RNA-seq transcriptional profiling of Herbaspirillum seropedicae colonizing wheat (Triticum... Herbaspirillum seropedicae is a diazotrophic and endophytic bacterium that associates with economically important grasses promoting plant growth and increasing productivity. To identify genes related to bacterial ability to colonize plants, wheat seedlings growing hydroponically in Hoagland’s medium were inoculated with H. seropedicae and incubated for 3 days. Total mRNA from the bacteria present in the root surface and in the plant medium were purified, depleted from rRNA and used for RNA-seq profiling. RT-qPCR analyses were conducted to confirm regulation of selected genes. Comparison of RNA profile of root attached and planktonic bacteria revealed extensive metabolic adaptations to the epiphytic life style. These adaptations include expression of specific adhesins and cell wall re-modeling to attach to the root. Additionally, the metabolism was adapted to the microxic environment and nitrogen-fixation genes were expressed. Polyhydroxybutyrate (PHB) synthesis was activated, and PHB granules were stored as observed by microscopy. Genes related to plant growth promotion, such as auxin production were expressed. Many ABC transporter genes were regulated in the bacteria attached to the roots. The results provide new insights into the adaptation of H. seropedicae to the interaction with the plant. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

RNA-seq transcriptional profiling of Herbaspirillum seropedicae colonizing wheat (Triticum aestivum) roots

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Publisher
Springer Journals
Copyright
Copyright © 2016 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-016-0430-6
Publisher site
See Article on Publisher Site

Abstract

Herbaspirillum seropedicae is a diazotrophic and endophytic bacterium that associates with economically important grasses promoting plant growth and increasing productivity. To identify genes related to bacterial ability to colonize plants, wheat seedlings growing hydroponically in Hoagland’s medium were inoculated with H. seropedicae and incubated for 3 days. Total mRNA from the bacteria present in the root surface and in the plant medium were purified, depleted from rRNA and used for RNA-seq profiling. RT-qPCR analyses were conducted to confirm regulation of selected genes. Comparison of RNA profile of root attached and planktonic bacteria revealed extensive metabolic adaptations to the epiphytic life style. These adaptations include expression of specific adhesins and cell wall re-modeling to attach to the root. Additionally, the metabolism was adapted to the microxic environment and nitrogen-fixation genes were expressed. Polyhydroxybutyrate (PHB) synthesis was activated, and PHB granules were stored as observed by microscopy. Genes related to plant growth promotion, such as auxin production were expressed. Many ABC transporter genes were regulated in the bacteria attached to the roots. The results provide new insights into the adaptation of H. seropedicae to the interaction with the plant.

Journal

Plant Molecular BiologySpringer Journals

Published: Jan 22, 2016

References

  • The role of root exudates in rhizosphere interactions with plants and other organisms
    Bais, HP; Weir, TL; Perry, LG; Gilroy, S; Vivanco, JM
  • Herbaspirillum seropedicae rfbB and rfbC genes are required for maize colonization
    Balsanelli, E; Serrato, RV; Baura, VA; Sassaki, G; Yates, MG; Rigo, LU; Pedrosa, FO; Souza, EM; Monteiro, RA
  • Nitrogen fixation control in Herbaspirillum seropedicae
    Chubatsu, LS; Monteiro, RA; Souza, EM; Oliveira, MAS; Yates, MG; Wassem, R; Bonatto, AC; Huergo, LF; Steffens, MBR; Rigo, LU; Pedrosa, FO
  • Use of plant colonizing bacteria as chassis for transfer of N2-fixation to cereals
    Geddes, BA; Ryu, MH; Mus, F; Garcia Costas, A; Peters, JW; Voigt, CA; Poole, P
  • Bacterial chemotaxis: the five sensors of a bacterium
    Grebe, TW; Stock, J

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