RNA Interference is Developmentally Regulated: Analysis of Expression of suffix, a Short Retroelement of Drosophila

RNA Interference is Developmentally Regulated: Analysis of Expression of suffix, a Short... Sense and antisense transcripts of suffix and F element were detected at different stages of Drosophila development. A short RNA, corresponding in size to the full-length suffix transcript, was also found. It was suggested that it could originate from the master copy of the element. Though transcription pattern of these elements changed during the development, transcription remained symmetrical. These data indicate that formation of dsRNA in the cells and triggering of the RNA interference mechanisms are possible at all stages of Drosophila development. Analysis of total RNA samples from all stages of Drosophila development showed that 21- to 25-nt long suffix-specific small interfering RNAs (siRNAs), the obligatory products of the RNA interference, are present only in pupae. Thereafter, RNA interference mechanisms are developmentally regulated and the formation of dsRNA is necessary but not sufficient for launching this potent and specific machinery of post-transcription silencing. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

RNA Interference is Developmentally Regulated: Analysis of Expression of suffix, a Short Retroelement of Drosophila

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2003 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/A:1022448313279
Publisher site
See Article on Publisher Site

Abstract

Sense and antisense transcripts of suffix and F element were detected at different stages of Drosophila development. A short RNA, corresponding in size to the full-length suffix transcript, was also found. It was suggested that it could originate from the master copy of the element. Though transcription pattern of these elements changed during the development, transcription remained symmetrical. These data indicate that formation of dsRNA in the cells and triggering of the RNA interference mechanisms are possible at all stages of Drosophila development. Analysis of total RNA samples from all stages of Drosophila development showed that 21- to 25-nt long suffix-specific small interfering RNAs (siRNAs), the obligatory products of the RNA interference, are present only in pupae. Thereafter, RNA interference mechanisms are developmentally regulated and the formation of dsRNA is necessary but not sufficient for launching this potent and specific machinery of post-transcription silencing.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 7, 2004

References

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