Rice black-streaked dwarf virus genome segment S5 is a bicistronic mRNA in infected plants

Rice black-streaked dwarf virus genome segment S5 is a bicistronic mRNA in infected plants Rice black-streaked dwarf virus (RBSDV) is a recognized member of the genus Fijivirus , family Reoviridae . Genome segment S5 has a putative second ORF partially overlapping the major ORF but in a different reading frame. This putative ORF is present in a published sequence and in two Chinese isolates now sequenced. Antibodies were raised against purified P5-1 and P5-2 fusion proteins expressed in a prokaryotic system. In western blots, these antibodies reacted with proteins of about 106 and 27 kDa, respectively, as predicted by sequence analysis. In immunoelectron microscopy, antibodies to P5-1 reacted with viroplasms, indicating that P5-1 is a component of viroplasms, but no labeling was observed with P5-2 antisera. Northern blot assays showed that the genome segment S5 was transcribed as a single mRNA with no subgenomic RNA. These results show that S5 is functionally bicistronic in infected plants. Possible translational mechanisms for P5-2 are discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Rice black-streaked dwarf virus genome segment S5 is a bicistronic mRNA in infected plants

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Springer Vienna
Copyright © 2014 by Springer-Verlag Wien
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
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  • Phylogenetic analysis reveals that a dwarfing disease on different cereal crops in China is due to rice black streaked dwarf virus (RBSDV)
    Bai, FW; Yan, J; Qu, ZC; Zhang, HW; Xu, J; Ye, MM; Shen, DL

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