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Rogério Margis, Emerson Reis, Vincent Villeret (1998)
Structural and phylogenetic relationships among plant and animal cystatins.Archives of biochemistry and biophysics, 359 1
S. Bustin, V. Beneš, J. Garson, J. Hellemans, J. Huggett, M. Kubista, Reinhold Mueller, T. Nolan, M. Pfaffl, G. Shipley, J. Vandesompele, C. Wittwer (2009)
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.Clinical chemistry, 55 4
S. Dutt, V. Singh, S. Marla, Anil Kumar (2010)
In silico Analysis of Sequential, Structural and Functional Diversity of Wheat Cystatins and Its Implication in Plant DefenseGenomics, Proteomics & Bioinformatics, 8
Manuel Martínez, M. Diaz-Mendoza, Laura Carrillo, I. Díaz (2007)
Carboxy terminal extended phytocystatins are bifunctional inhibitors of papain and legumain cysteine proteinasesFEBS Letters, 581
Bruno Printz, R. Morais, S. Wienkoop, K. Sergeant, S. Lutts, J. Hausman, J. Renaut (2015)
An improved protocol to study the plant cell wall proteomeFrontiers in Plant Science, 6
Xiaoyu Qiang, B. Zechmann, Marco Reitz, K. Kogel, P. Schäfer (2012)
The Mutualistic Fungus Piriformospora indica Colonizes Arabidopsis Roots by Inducing an Endoplasmic Reticulum Stress–Triggered Caspase-Dependent Cell Death[C][W]Plant Cell, 24
HM Berman (2000)
The protein data bankNucleic Acids Res, 28
M Benchabane, U Schlüter, J Vorster, M-C Goulet, D Michaud (2010)
Plant cystatinsBiochimie, 92
J. Hwang, J. Hong, Jihyun Je, Kyun Lee, Dool-Yi Kim, Sang Lee, C. Lim (2009)
Regulation of seed germination and seedling growth by an Arabidopsis phytocystatin isoform, AtCYS6Plant Cell Reports, 28
K. Abe, Y. Emori, H. Kondo, S. Arai, K. Suzuki (1988)
The NH2-terminal 21 amino acid residues are not essential for the papain-inhibitory activity of oryzacystatin, a member of the cystatin superfamily. Expression of oryzacystatin cDNA and its truncated fragments in Escherichia coli.The Journal of biological chemistry, 263 16
Bing-Sheng Lv, Liang Xiaowei, Hong-yuan Ma, Yang Sun, Wei Lixing, Chang-Jie Jiang, Zhengwei Liang (2013)
Differences in Growth and Physiology of Rice in Response to Different Saline-Alkaline Stress FactorsAgronomy Journal, 105
K. Tamura, G. Stecher, D. Peterson, A. Filipski, Sudhir Kumar (2013)
MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.Molecular biology and evolution, 30 12
R. Jefferson, T. Kavanagh, M. Bevan (1987)
GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.The EMBO Journal, 6
K. Kunert, S. Wyk, C. Cullis, B. Vorster, C. Foyer (2015)
Potential use of phytocystatins in crop improvement, with a particular focus on legumes.Journal of experimental botany, 66 12
J. Hong, J. Hwang, Chan Lim, K. Yang, Z. Jin, Cha Kim, J. Koo, W. Chung, Kyun Lee, Sang Lee, M. Cho, C. Lim (2007)
Over-expression of Chinese cabbage phytocystatin 1 retards seed germination in ArabidopsisPlant Science, 172
Manuel Martínez, Zamira Abraham, P. Carbonero, I. Díaz (2005)
Comparative phylogenetic analysis of cystatin gene families from arabidopsis, rice and barleyMolecular Genetics and Genomics, 273
Manuel Martínez, Zamira Abraham, M. Gambardella, M. Echaide, P. Carbonero, I. Díaz (2005)
The strawberry gene Cyf1 encodes a phytocystatin with antifungal properties.Journal of experimental botany, 56 417
S. Parasuraman (2012)
Protein data bankJournal of Pharmacology & Pharmacotherapeutics, 3
M. Margis-Pinheiro, Andreia Zolet, Guilherme Loss, G. Pasquali, R. Margis (2008)
Molecular evolution and diversification of plant cysteine proteinase inhibitors: new insights after the poplar genome.Molecular phylogenetics and evolution, 49 1
M. Lescot, P. Déhais, G. Thijs, K. Marchal, Y. Moreau, Y. Peer, P. Rouzé, S. Rombauts (2002)
PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequencesNucleic acids research, 30 1
Marko Novinec, B. Lenarčič (2013)
Papain-like peptidases: structure, function, and evolution, 4
R. Hoorn (2008)
Plant proteases: from phenotypes to molecular mechanisms.Annual review of plant biology, 59
G. Agrawal, R. Rakwal, S. Tamogami, M. Yonekura, A. Kubo, H. Saji (2002)
Chitosan activates defense/stress response(s) in the leaves of Oryza sativa seedlingsPlant Physiology and Biochemistry, 40
Ke-Ming Wang, Senthil Kumar, Yi-Sheng Cheng, Shripathi Venkatagiri, A. Yang, K. Yeh (2008)
Characterization of inhibitory mechanism and antifungal activity between group‐1 and group‐2 phytocystatins from taro (Colocasia esculenta)The Febs Journal, 275
Wen-Chi Chang, Tzong-Yi Lee, Hsien-Da Huang, His-Yuan Huang, Rong-Long Pan (2008)
PlantPAN: Plant promoter analysis navigator, for identifying combinatorial cis-regulatory elements with distance constraint in plant gene groupsBMC Genomics, 9
H. Watanabe, K. Abe, Y. Emori, Hiroshi Hosoyama, S. Arai (1991)
Molecular cloning and gibberellin-induced expression of multiple cysteine proteinases of rice seeds (oryzains).The Journal of biological chemistry, 266 25
Laura Carrillo, I. Herrero, Inés Cambra, R. Sánchez-Monge, I. Díaz, Manuel Martínez (2011)
Differential in vitro and in vivo effect of barley cysteine and serine protease inhibitors on phytopathogenic microorganisms.Plant physiology and biochemistry : PPB, 49 10
A. Christoff, R. Margis (2014)
The diversity of rice phytocystatinsMolecular Genetics and Genomics, 289
K. Müntz, F. Blattner, A. Shutov (2002)
Legumains - a family of asparagine-specific cysteine endopeptidases involved in propolypeptide processing and protein breakdown in plantsJournal of Plant Physiology, 159
S. Arai, H. Watanabe, H. Kondo, Y. Emori, K. Abe (1991)
Papain-inhibitory activity of oryzacystatin, a rice seed cysteine proteinase inhibitor, depends on the central Gln-Val-Val-Ala-Gly region conserved among cystatin superfamily members.Journal of biochemistry, 109 2
K. Livak, Thomas Schmittgen (2001)
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.Methods, 25 4
Peijian Cao, K. Jung, Daeseok Choi, D. Hwang, Jun Zhu, P. Ronald (2012)
The Rice Oligonucleotide Array Database: an atlas of rice gene expressionRice, 5
J. Hwang, J. Hong, Chan Lim, Huan Chen, Jihyun Je, K. Yang, Dool-Yi Kim, Y. Choi, Sang Lee, C. Lim (2010)
Distinct expression patterns of two Arabidopsis phytocystatin genes, AtCYS1 and AtCYS2, during development and abiotic stressesPlant Cell Reports, 29
N. Hatsugai, M. Kuroyanagi, Kenji Yamada, T. Meshi, S. Tsuda, M. Kondo, M. Nishimura, I. Hara-Nishimura (2004)
A Plant Vacuolar Protease, VPE, Mediates Virus-Induced Hypersensitive Cell DeathScience, 305
J. Vandesompele, K. Preter, F. Pattyn, B. Poppe, N. Roy, A. Paepe, F. Speleman (2002)
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genesGenome Biology, 3
Mei-Ling Cheng, J. Tzen, D. Shyu, W. Chou (2014)
Functional characterization of the N-terminal and C-terminal domains of a sesame group II phytocystatinBotanical Studies, 55
J. Hong, J. Hwang, W. Chung, Kyun Lee, Y. Choi, S. Gal, Beom-Seok Park, C. Lim (2008)
Expression of a Chinese cabbage cysteine proteinase inhibitor, BrCYS1, retards seed germination and plant growth in transgenic Tobacco plantJournal of Plant Biology, 51
S. Ohtsubo, Hitoshi Kobayashi, W. Noro, M. Taniguchi, E. Saitoh (2005)
Molecular cloning and characterization of oryzacystatin-III, a novel member of phytocystatin in rice (Oryza sativa L. japonica).Journal of agricultural and food chemistry, 53 13
B. Belenghi, F. Acconcia, M. Trovato, M. Perazzolli, A. Bocedi, F. Polticelli, P. Ascenzi, M. Delledonne (2003)
AtCYS1, a cystatin from Arabidopsis thaliana, suppresses hypersensitive cell death.European journal of biochemistry, 270 12
T. Komari, Y. Ishida, Yukoh Hiei (2009)
Transgenic Rice
M. Diaz-Mendoza, Blanca Velasco-Arroyo, P. González-Melendi, Manuel Martínez, I. Díaz (2014)
C1A cysteine protease-cystatin interactions in leaf senescence.Journal of experimental botany, 65 14
Sanjay Kumar, Xiaodong Cheng, S. Klimašauskas, S. Mi, J. Posfai, R. Roberts, G. Wilson (1994)
The DNA (cytosine-5) methyltransferasesNucleic acids research, 22 1
L. Kelley, Stefans Mezulis, C. Yates, M. Wass, M. Sternberg (2015)
The Phyre2 web portal for protein modeling, prediction and analysisNature Protocols, 10
J. Ruijter, Christian Ramakers, W. Hoogaars, Y. Karlen, O. Bakker, M. Hoff, A. Moorman (2009)
Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR dataNucleic Acids Research, 37
Xiaoli Sun, Shanshan Yang, Mingzhe Sun, Sunting Wang, Xiaodong Ding, Dan Zhu, W. Ji, H. Cai, Chaoyue Zhao, Xuedong Wang, Yanming Zhu (2014)
A novel Glycine soja cysteine proteinase inhibitor GsCPI14, interacting with the calcium/calmodulin-binding receptor-like kinase GsCBRLK, regulated plant tolerance to alkali stressPlant Molecular Biology, 85
E. Pesquet (2012)
Plant proteases - from detection to function.Physiologia plantarum, 145 1
C. Pirovani, André Santiago, L. Santos, F. Micheli, R. Margis, Abelmon Gesteira, F. Alvim, G. Pereira, Júlio Cascardo (2010)
Theobroma cacao cystatins impair Moniliophthora perniciosa mycelial growth and are involved in postponing cell death symptomsPlanta, 232
W. Bode, R. Engh, D. Musil, U. Thiele, R. Huber, Andrej Karshikovl, J. Brzin, J. Kos, V. Turk (1988)
The 2.0 A X‐ray crystal structure of chicken egg white cystatin and its possible mode of interaction with cysteine proteinases.The EMBO Journal, 7
S. Arai, I. Matsumoto, Y. Emori, K. Abe (2002)
Plant seed cystatins and their target enzymes of endogenous and exogenous origin.Journal of agricultural and food chemistry, 50 22
K Abe, Y Emori, H Kondo, S Arai, K Suzuki (1988)
The NH2-terminal 21 amino acid residues are not essential for the papain-inhibitory activity of oryzacystatin, a member of the cystatin superfamilyJ Biol Chem, 263
Olivier Pierre, Julie Hopkins, M. Combier, F. Baldacci, G. Engler, R. Brouquisse, D. Hérouart, E. Boncompagni (2014)
Involvement of papain and legumain proteinase in the senescence process of Medicago truncatula nodules.The New phytologist, 202 3
M. Chu, Kai-Lun Liu, Hsin‐Yi Wu, K. Yeh, Yi-Sheng Cheng (2011)
Crystal structure of tarocystatin–papain complex: implications for the inhibition property of group-2 phytocystatinsPlanta, 234
T. Okamoto, A. Yuki, N. Mitsuhashi, Takao Mimamikawa (1999)
Asparaginyl endopeptidase (VmPE-1) and autocatalytic processing synergistically activate the vacuolar cysteine proteinase (SH-EP).European journal of biochemistry, 264 1
R. Senthilkumar, Chiu-Ping Cheng, K. Yeh (2010)
Genetically pyramiding protease-inhibitor genes for dual broad-spectrum resistance against insect and phytopathogens in transgenic tobacco.Plant biotechnology journal, 8 1
Maud Lepelley, M. Amor, Nelly Martineau, Gerald Cheminade, Victoria Caillet, J. McCarthy (2012)
Coffee cysteine proteinases and related inhibitors with high expression during grain maturation and germinationBMC Plant Biology, 12
(2016)
Plant Mol Biol
K. Nicholas, H. Nicholas (1997)
GeneDoc: a tool for editing and annotating multiple sequence alignments
Laura Carrillo, Manuel Martínez, K. Ramessar, Inés Cambra, P. Castanera, F. Ortego, I. Díaz (2010)
Expression of a barley cystatin gene in maize enhances resistance against phytophagous mites by altering their cysteine-proteasesPlant Cell Reports, 30
N. Hatsugai, Kenji Yamada, Shino Goto-Yamada, I. Hara-Nishimura (2015)
Vacuolar processing enzyme in plant programmed cell deathFrontiers in Plant Science, 6
Xinxin Zhang, Shenkui Liu, T. Takano (2008)
Two cysteine proteinase inhibitors from Arabidopsis thaliana, AtCYSa and AtCYSb, increasing the salt, drought, oxidation and cold tolerancePlant Molecular Biology, 68
M. Karimi, D. Inzé, A. Depicker (2002)
GATEWAY vectors for Agrobacterium-mediated plant transformation.Trends in plant science, 7 5
M. Álvarez-Fernández, A. Barrett, B. Gerhartz, P. Dando, J. Ni, M. Abrahamson (1999)
Inhibition of Mammalian Legumain by Some Cystatins Is Due to a Novel Second Reactive Site*The Journal of Biological Chemistry, 274
J. Thompson, D. Higgins, T. Gibson (1994)
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.Nucleic acids research, 22 22
S. Valdés‐Rodríguez, Armando Guerrero-Rangel, C. Melgoza-Villagómez, A. Chagolla-López, F. Delgado-Vargas, N. Martínez-Gallardo, C. Sánchez-Hernández, J. Délano-Frier (2007)
Cloning of a cDNA encoding a cystatin from grain amaranth (Amaranthus hypochondriacus) showing a tissue-specific expression that is modified by germination and abiotic stress.Plant physiology and biochemistry : PPB, 45 10-11
A. Christoff, A. Turchetto-Zolet, R. Margis (2014)
Uncovering legumain genes in rice.Plant science : an international journal of experimental plant biology, 215-216
M. Quain, M. Makgopa, Belen Marquez-Garcia, G. Comadira, N. Fernandez‐Garcia, E. Olmos, D. Schnaubelt, K. Kunert, C. Foyer (2014)
Ectopic phytocystatin expression leads to enhanced drought stress tolerance in soybean (Glycine max) and Arabidopsis thaliana through effects on strigolactone pathways and can also result in improved seed traits.Plant biotechnology journal, 12 7
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
H. Kato, Takao Mtnamikawa (1996)
Identification and characterization of a rice cysteine endopeptidase that digests glutelin.European journal of biochemistry, 239 2
A. Mikkonen, Ilkka Porali, M. Cercós, T. Ho (1996)
A major cysteine proteinase, EPB, in germinating barley seeds: structure of two intronless genes and regulation of expressionPlant Molecular Biology, 31
Ningyan Zhang, Berne Jones (2004)
Purification and partial characterization of a 31-kDa cysteine endopeptidase from germinated barleyPlanta, 199
D. Miki, K. Shimamoto (2004)
Simple RNAi vectors for stable and transient suppression of gene function in rice.Plant & cell physiology, 45 4
Wided Megdiche, C. Passaquet, Walid Zourrig, Yasmine Fodil, C. Abdelly (2009)
Molecular cloning and characterization of novel cystatin gene in leaves Cakile maritima halophyte.Journal of plant physiology, 166 7
A. Yang, A. Yang, Kai-Wun Yeh (2005)
Molecular cloning, recombinant gene expression, and antifungal activity of cystatin from taro (Colocasia esculenta cv. Kaosiung no. 1)Planta, 221
D. Goodstein, S. Shu, R. Howson, R. Neupane, Richard Hayes, Joni Fazo, T. Mitros, W. Dirks, U. Hellsten, Nicholas Putnam, D. Rokhsar (2011)
Phytozome: a comparative platform for green plant genomicsNucleic Acids Research, 40
Manuel Martínez, Inés Cambra, Laura Carrillo, M. Diaz-Mendoza, I. Díaz (2009)
Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination1[W]Plant Physiology, 151
N Upadhyaya, X Zhou, Q Zhu, A Eamens, M Wang, P Waterhouse, E Dennis (2002)
Transgenic cereals
Phytocystatins are well-known inhibitors of C1A cysteine proteinases. However, previous research has revealed legumain (C13) protease inhibition via a carboxy-extended phytocystatin. Among the 12 phytocystatins genes in rice, OcXII is the only gene possessing this carboxy-terminal extension. The specific legumain inhibition activity was confirmed, in our work, using a recombinant OcXII harboring only the carboxy-terminal domain and this part did not exhibit any effect on papain-like activities. Meanwhile, rice plants silenced at the whole OcXII gene presented higher legumain and papain-like proteolytic activities, resulting in a faster initial seedling growth. However, when germinated under stressful alkaline conditions, OcXII-silenced plants exhibited impaired root formation and delayed shoot growth. Interestingly, the activity of OcXII promoter gene was detected in the rice seed scutellum region, and decreases with seedling growth. Seeds from these plants also exhibited slower growth at germination under ABA or alkaline conditions, while maintaining very high levels of OcXII transcriptional activation. This likely reinforces the proteolytic control necessary for seed germination and growth. In addition, increased legumain activity was detected in OcXII RNAi plants subjected to a fungal elicitor. Overall, the results of this study highlight the association of OcXII with not only plant development processes, but also with stress response pathways. The results of this study reinforce the bifunctional ability of carboxy-extended phytocystatins in regulating legumain proteases via its carboxy-extended domain and papain-like proteases by its amino-terminal domain.
Plant Molecular Biology – Springer Journals
Published: Jun 20, 2016
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