Rht23 (5Dq′) likely encodes a Q homeologue with pleiotropic effects on plant height and spike compactness

Rht23 (5Dq′) likely encodes a Q homeologue with pleiotropic effects on plant height and spike... The domesticated gene Q on wheat chromosome 5A (5AQ) encodes an AP2 transcription factor. The 5AQ was originated from a G to A mutation in exon 8 and/or C to T transition in exon 10 and resulted in free-threshing and subcompact spike characters of bread wheat. The Q homeoalleles on 5B and 5D are either a pseudogene or expressed at a low level. Our previ- ous study identified a mutant, named NAUH164, by EMS treatment of wheat variety Sumai 3. The mutant exhibits compact spike and dwarfness, and the mutated locus Rht23 was mapped to the distal of the long arm of chromosome 5D, where 5Dq was located. To investigate the relationship of Rht23 and 5Dq, sequences and expression patterns of 5Dq from Sumai 3 and NAUH164 were compared. The two genotypes had a G3147A single nucleotide polymorphism (SNP), which was predicted to be located within the miR172 binding site of 5Dq. Based on this SNP, an SNP marker was developed and linkage analysis using a (NAUH164 × Alondra’s) RIL population showed the marker was co-segregated with the Rht23 mutant traits. The qRT- PCR and Northern blot showed that in NAUH164, the expression of 5Dq was significantly up-regulated, and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png TAG Theoretical and Applied Genetics Springer Journals

Rht23 (5Dq′) likely encodes a Q homeologue with pleiotropic effects on plant height and spike compactness

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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2018 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Life Sciences; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Agriculture; Plant Biochemistry; Biochemistry, general; Biotechnology
ISSN
0040-5752
eISSN
1432-2242
D.O.I.
10.1007/s00122-018-3115-5
Publisher site
See Article on Publisher Site

Abstract

The domesticated gene Q on wheat chromosome 5A (5AQ) encodes an AP2 transcription factor. The 5AQ was originated from a G to A mutation in exon 8 and/or C to T transition in exon 10 and resulted in free-threshing and subcompact spike characters of bread wheat. The Q homeoalleles on 5B and 5D are either a pseudogene or expressed at a low level. Our previ- ous study identified a mutant, named NAUH164, by EMS treatment of wheat variety Sumai 3. The mutant exhibits compact spike and dwarfness, and the mutated locus Rht23 was mapped to the distal of the long arm of chromosome 5D, where 5Dq was located. To investigate the relationship of Rht23 and 5Dq, sequences and expression patterns of 5Dq from Sumai 3 and NAUH164 were compared. The two genotypes had a G3147A single nucleotide polymorphism (SNP), which was predicted to be located within the miR172 binding site of 5Dq. Based on this SNP, an SNP marker was developed and linkage analysis using a (NAUH164 × Alondra’s) RIL population showed the marker was co-segregated with the Rht23 mutant traits. The qRT- PCR and Northern blot showed that in NAUH164, the expression of 5Dq was significantly up-regulated, and

Journal

TAG Theoretical and Applied GeneticsSpringer Journals

Published: May 31, 2018

References

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