Mammalian Genome 12, 799–801 (2001). DOI: 10.1007/s003350020043 Incorporating Mouse Genome © Springer-Verlag New York Inc. 2001 RH mapping of canine TOAST markers: a new strategy for species-specific primer design to prevent amplification of host orthologous gene products especially with similar sizes 1,2 1, 1 1 Zhihua Jiang, * Corinne Renier, * Catherine Andre ´, Francis Galibert UMR 6061 CNRS “Ge ´ne ´tique et De ´veloppement”, Faculte´de Me ´decine, 2 avenue du Professeur Le ´on Bernard, 35043 Rennes Cedex, France Centre for Genetic Improvement of Livestock, Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1 Received: 29 December 2000 / Accepted: 11 June 2001 The high degree of sequence conservation between orthologous TOAST markers on dog-hamster radiation hybrid cell lines by genes has allowed development of reagents for mammalian com- developing species-specific primers. Twenty-eight canine parative gene mapping and amplification of orthologous genes in TOASTs that have not been mapped on the RH map with the different species. Traced orthologous amplified sequence tags gene-specific universal primers owing to co-amplification of ham- (TOASTs; Jiang et al. 1998), comparative anchor-tagged se- ster orthologous gene products with similar sizes were selected for quences (CATS; Lyons et al. 1997),
Mammalian Genome – Springer Journals
Published: Feb 20, 2014
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