Reversed-Phase HPLC Method for Determination of Temozolomide in Rat Plasma and Brain: Simple, Sensitive and Robust Method

Reversed-Phase HPLC Method for Determination of Temozolomide in Rat Plasma and Brain: Simple,... A simple and sensitive HPLC method was developed and validated to detect an anti-glioma drug (temozolomide) in rat plasma and brain. The drug and internal standard (metronidazole) were eluted at proper retention times using BDS C18 column and selected mobile phase (ammonium formate – acetonitrile). The proposed method showed specificity and linearity with R 2 values of 0.9964 and 0.9978 in plasma and brain, respectively. Other parameters such as intraday and interday precision were found to be within acceptable limits. The LOD and LOQ were 84 and 255 ng/mL, respectively. The drug recovery from the spiked plasma varied from 43 to 52%. Furthermore, stability of the method was evaluated and it was found that the drug exhibited good stability except at room temperature. The percentage recovery at room temperature after 24 h was within 69 – 73%; however, the drug was stable up to 15 days at –70°C. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Pharmaceutical Chemistry Journal Springer Journals

Reversed-Phase HPLC Method for Determination of Temozolomide in Rat Plasma and Brain: Simple, Sensitive and Robust Method

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Pharmacy; Pharmacy; Pharmacology/Toxicology; Organic Chemistry
ISSN
0091-150X
eISSN
1573-9031
D.O.I.
10.1007/s11094-018-1804-7
Publisher site
See Article on Publisher Site

Abstract

A simple and sensitive HPLC method was developed and validated to detect an anti-glioma drug (temozolomide) in rat plasma and brain. The drug and internal standard (metronidazole) were eluted at proper retention times using BDS C18 column and selected mobile phase (ammonium formate – acetonitrile). The proposed method showed specificity and linearity with R 2 values of 0.9964 and 0.9978 in plasma and brain, respectively. Other parameters such as intraday and interday precision were found to be within acceptable limits. The LOD and LOQ were 84 and 255 ng/mL, respectively. The drug recovery from the spiked plasma varied from 43 to 52%. Furthermore, stability of the method was evaluated and it was found that the drug exhibited good stability except at room temperature. The percentage recovery at room temperature after 24 h was within 69 – 73%; however, the drug was stable up to 15 days at –70°C.

Journal

Pharmaceutical Chemistry JournalSpringer Journals

Published: Jun 1, 2018

References

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