RETRACTED ARTICLE: Brassica RNA binding protein ERD4 is involved in conferring salt, drought tolerance and enhancing plant growth in Arabidopsis

RETRACTED ARTICLE: Brassica RNA binding protein ERD4 is involved in conferring salt, drought... ‘Early responsive to dehydration’ (ERD) genes are a group of plant genes having functional roles in plant stress tolerance and development. In this study, we have isolated and characterized a Brassica juncea ‘ERD’ gene (BjERD4) which encodes a novel RNA binding protein. The expression pattern of ERD4 analyzed under different stress conditions showed that transcript levels were increased with dehydration, sodium chloride, low temperature, heat, abscisic acid and salicylic acid treatments. The BjERD4 was found to be localized in the chloroplasts as revealed by Confocal microscopy studies. To study the function, transgenic Arabidopsis plants were generated and analyzed for various morphological and physiological parameters. The overexpressing transgenic lines showed significant increase in number of leaves with more leaf area and larger siliques as compared to wild type plants, whereas RNAi:ERD4 transgenic lines showed reduced leaf number, leaf area, dwarf phenotype and delayed seed germination. Transgenic Arabidopsis plants overexpressing BjERD4 gene also exhibited enhanced tolerance to dehydration and salt stresses, while the knockdown lines were susceptible as compared to wild type plants under similar stress conditions. It was observed that BjERD4 protein could bind RNA as evidenced by the gel-shift assay. The overall results of transcript analysis, RNA gel-shift assay, and transgenic expression, for the first time, show that the BjERD4 is involved in abiotic stress tolerance besides offering new clues about the possible roles of BjERD4 in plant growth and development. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

RETRACTED ARTICLE: Brassica RNA binding protein ERD4 is involved in conferring salt, drought tolerance and enhancing plant growth in Arabidopsis

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Publisher
Springer Netherlands
Copyright
Copyright © 2015 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-015-0423-x
Publisher site
See Article on Publisher Site

Abstract

‘Early responsive to dehydration’ (ERD) genes are a group of plant genes having functional roles in plant stress tolerance and development. In this study, we have isolated and characterized a Brassica juncea ‘ERD’ gene (BjERD4) which encodes a novel RNA binding protein. The expression pattern of ERD4 analyzed under different stress conditions showed that transcript levels were increased with dehydration, sodium chloride, low temperature, heat, abscisic acid and salicylic acid treatments. The BjERD4 was found to be localized in the chloroplasts as revealed by Confocal microscopy studies. To study the function, transgenic Arabidopsis plants were generated and analyzed for various morphological and physiological parameters. The overexpressing transgenic lines showed significant increase in number of leaves with more leaf area and larger siliques as compared to wild type plants, whereas RNAi:ERD4 transgenic lines showed reduced leaf number, leaf area, dwarf phenotype and delayed seed germination. Transgenic Arabidopsis plants overexpressing BjERD4 gene also exhibited enhanced tolerance to dehydration and salt stresses, while the knockdown lines were susceptible as compared to wild type plants under similar stress conditions. It was observed that BjERD4 protein could bind RNA as evidenced by the gel-shift assay. The overall results of transcript analysis, RNA gel-shift assay, and transgenic expression, for the first time, show that the BjERD4 is involved in abiotic stress tolerance besides offering new clues about the possible roles of BjERD4 in plant growth and development.

Journal

Plant Molecular BiologySpringer Journals

Published: Dec 28, 2015

References

  • Novel perspectives for the engineering of abiotic stress tolerance in plants
    Cabello, JV; Lodeyro, AF; Zurbriggen, MD
  • Identification of genes responsive to the application of ethanol on sugarcane leaves
    Camargo, SR; Cancado, GMA; Ulian, EC; Menossi, M
  • Abnormal chloroplast development and growth inhibition in rice thioredoxin m knock-down plants
    Chi, YH; Moon, JC; Park, JH
  • Comprehensive functional analysis of the catalase gene family in Arabidopsis thaliana
    Du, Y; Wang, P; Chen, J; Song, CP
  • Physiological roles of ERD10 in abiotic stresses and seed germination of Arabidopsis
    Kim, SY; Nam, KH
  • ERD6, a cDNA clone for an early dehydration-induced gene of Arabidopsis, encodes a putative sugar transporter
    Kiyosue, T; Abe, H; Yamaguchi-Shinozaki, K; Shinozaki, K
  • Chaperon activity of ERD10 and ERD14, two disordered stress-related plant proteins
    Kovacs, D; Kalmar, E; Torok, Z; Tompa, P
  • Both the hydrophobicity and a positively charged region flanking the C-terminal region of the transmembrane domain of signal-anchored proteins play critical roles in determining their targeting specificity to the endoplasmic reticulum or endosymbiotic organelles in Arabidopsis cells
    Lee, J; Lee, H; Kim, J; Lee, S; Kim, DH; Kim, S; Hwang, I
  • A maize early responsive to dehydration gene, ZmERD4, provides enhanced drought and salt tolerance in Arabidopsis
    Liu, Y; Li, H; Shi, Y; Song, Y; Wang, T; Li, Y

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