Results of Camelus dromedarius and Camelus bactrianus Genotyping by Alpha-S1-Casein, Kappa-Casein Loci, and DNA Fingerprinting

Results of Camelus dromedarius and Camelus bactrianus Genotyping by Alpha-S1-Casein, Kappa-Casein... Genotyping of Kazakh camels Camelus dromedarius (milk breed) (n = 18) and Camelus bactrianus (meat breed) (n = 18) by alpha-S1-casein (αs1-CN) and kappa-casein (κ-CN) loci was conducted using the PCR–RFLP analysis method. A new pair of primers was suggested for the amplification of the CSN3 gene fragment with subsequent cleavage of the reaction products by AluI restriction endonuclease in order to identify the gene genetic variants. DNA polymorphism was detected only for the kappa-casein locus; no genetic polymorphism for alpha-S1-casein gene was found in the studied populations. Analysis of the results of DNA fingerprinting demonstrated that the band sharing (BS) coefficient between the groups was low enough (0.13), and the genetic distance (D) between Dromedary and Bactrian breeds was 0.305. The results of genotyping of Bactrian and Dromedary Kazakh camel breeds by alpha-S1-casein, kappa-casein loci, and DNA fingerprinting indicate that the Dromedary breed female camels are more polymorphic as compared with Bactrian. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Cytology and Genetics Springer Journals

Results of Camelus dromedarius and Camelus bactrianus Genotyping by Alpha-S1-Casein, Kappa-Casein Loci, and DNA Fingerprinting

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Publisher
Pleiades Publishing
Copyright
Copyright © 2018 by Allerton Press, Inc.
Subject
Biomedicine; Human Genetics
ISSN
0095-4527
eISSN
1934-9440
D.O.I.
10.3103/S0095452718030040
Publisher site
See Article on Publisher Site

Abstract

Genotyping of Kazakh camels Camelus dromedarius (milk breed) (n = 18) and Camelus bactrianus (meat breed) (n = 18) by alpha-S1-casein (αs1-CN) and kappa-casein (κ-CN) loci was conducted using the PCR–RFLP analysis method. A new pair of primers was suggested for the amplification of the CSN3 gene fragment with subsequent cleavage of the reaction products by AluI restriction endonuclease in order to identify the gene genetic variants. DNA polymorphism was detected only for the kappa-casein locus; no genetic polymorphism for alpha-S1-casein gene was found in the studied populations. Analysis of the results of DNA fingerprinting demonstrated that the band sharing (BS) coefficient between the groups was low enough (0.13), and the genetic distance (D) between Dromedary and Bactrian breeds was 0.305. The results of genotyping of Bactrian and Dromedary Kazakh camel breeds by alpha-S1-casein, kappa-casein loci, and DNA fingerprinting indicate that the Dromedary breed female camels are more polymorphic as compared with Bactrian.

Journal

Cytology and GeneticsSpringer Journals

Published: Jun 4, 2018

References

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