Respiration: comparison of the Winkler technique, O2 electrodes, O2 optodes and the respiratory electron transport system assay

Respiration: comparison of the Winkler technique, O2 electrodes, O2 optodes and the respiratory... Aerobic respiration is a biological energy generation process that consumes organic carbon and oxygen. In the ocean, the balance between photosynthesis and respiration is recognized as critical to understanding the ocean’s impact on the hydrospheric and atmospheric CO2. Techniques to determine respiration can be based on inorganic chemistry, electrochemistry, photochemistry, and enzymology. Here, for method comparison, physiological respiration was simultaneously measured by the Winkler method (W), O2 electrodes (E), and O2 optodes (O). These techniques detected respiratory O2 consumption (R), in situ, in dark incubation chambers. Respiratory electron transport system activity measurements detected potential respiration (Ф), biochemically. Leptomysis lingvura, a marine mysid, and Ulva rigida, a species of green algal sea lettuce, were the two organisms tested. Physiological respiration results from each technique were not statistically significantly different (multiple paired Student’s t tests, p value > 0.05) and were inside the range of similar published measurements. The mean dry-mass-specific respiration in L. lingvura and U. rigida was 0.147 ± 0.037 and 0.023 ± 0.008 µmol O2 h−1 (mg dry mass)−1, n = 9, respectively. The R-to-Ф ratios were different in the two organisms. However, linear regression between R and Ф for L. lingvura and U. rigida was stronger (r 2 = 0.814 and 0.313) than the linear regression between R and dry biomass (r 2 = 0.643 and 0.213). The application of Passing–Bablok regression analysis evidenced the high correlation between the results, and the Bland–Altman analysis examined the average difference (“bias”) and limits of agreement between the methods. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Marine Biology Springer Journals

Respiration: comparison of the Winkler technique, O2 electrodes, O2 optodes and the respiratory electron transport system assay

Loading next page...
 
/lp/springer_journal/respiration-comparison-of-the-winkler-technique-o2-electrodes-o2-rKzJc0XjHr
Publisher
Springer Journals
Copyright
Copyright © 2017 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Environment; Marine & Freshwater Sciences; Freshwater & Marine Ecology; Oceanography; Microbiology; Zoology
ISSN
0025-3162
eISSN
1432-1793
D.O.I.
10.1007/s00227-017-3271-1
Publisher site
See Article on Publisher Site

Abstract

Aerobic respiration is a biological energy generation process that consumes organic carbon and oxygen. In the ocean, the balance between photosynthesis and respiration is recognized as critical to understanding the ocean’s impact on the hydrospheric and atmospheric CO2. Techniques to determine respiration can be based on inorganic chemistry, electrochemistry, photochemistry, and enzymology. Here, for method comparison, physiological respiration was simultaneously measured by the Winkler method (W), O2 electrodes (E), and O2 optodes (O). These techniques detected respiratory O2 consumption (R), in situ, in dark incubation chambers. Respiratory electron transport system activity measurements detected potential respiration (Ф), biochemically. Leptomysis lingvura, a marine mysid, and Ulva rigida, a species of green algal sea lettuce, were the two organisms tested. Physiological respiration results from each technique were not statistically significantly different (multiple paired Student’s t tests, p value > 0.05) and were inside the range of similar published measurements. The mean dry-mass-specific respiration in L. lingvura and U. rigida was 0.147 ± 0.037 and 0.023 ± 0.008 µmol O2 h−1 (mg dry mass)−1, n = 9, respectively. The R-to-Ф ratios were different in the two organisms. However, linear regression between R and Ф for L. lingvura and U. rigida was stronger (r 2 = 0.814 and 0.313) than the linear regression between R and dry biomass (r 2 = 0.643 and 0.213). The application of Passing–Bablok regression analysis evidenced the high correlation between the results, and the Bland–Altman analysis examined the average difference (“bias”) and limits of agreement between the methods.

Journal

Marine BiologySpringer Journals

Published: Dec 2, 2017

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off