Resistance of microalgae to colloidal silver nanoparticles

Resistance of microalgae to colloidal silver nanoparticles Effect of colloidal silver nanoparticles on growth and structure of laboratory populations was investigated in chlorococcal algae Scenedesmus quadricauda (Turp.) Bréb. and Monoraphidium arcuatum (Korsch.) Hind. in the range of concentrations from 0.0001 to 1 mg/L. The toxicity of colloidal silver was manifested at a concentration of 0.1 mg/L and higher and was associated with an algostatic effect, which duration directly depended on silver concentration in the medium. After a period of growth suppression at a concentration of 0.1 mg/L, in both species cell population in the culture was gradually recovered and the structure of population (ratio between the fractions of dividing, quiescent, and dead cells) became the same as in control material. The effect of adaptation of S. quadricauda to colloidal silver was detected upon successive passages to the media with toxic concentrations (0.1 and 0.5 mg/L), which depended on the concentration and duration of initial intoxication of the culture. The toxicity of cell-free filtrates from the culture of S. quadricauda, which was in contact with colloidal silver for different time periods, was estimated using intact culture of the same species, whose population on cell-free filtrates was close to control level. Transfer of the cells that were exposed to silver to a pure medium led to a long lag phase in all the cases. It is assumed that the main mechanism of algal population adaptation to higher concentrations (0.1 and 0.5 mg/L) of this toxicant depends on the selection of resistant cells present in the initial heterogeneous population in the course of primary exposure to colloidal silver. These resistant cells accidentally emerge in the population by means of rare spontaneous mutations occurring in the course of replication even before the contact with silver and have more perfect mechanisms of its detoxification. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Resistance of microalgae to colloidal silver nanoparticles

Loading next page...
 
/lp/springer_journal/resistance-of-microalgae-to-colloidal-silver-nanoparticles-saVAPJ6xJk
Publisher
Pleiades Publishing
Copyright
Copyright © 2015 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443715010082
Publisher site
See Article on Publisher Site

Abstract

Effect of colloidal silver nanoparticles on growth and structure of laboratory populations was investigated in chlorococcal algae Scenedesmus quadricauda (Turp.) Bréb. and Monoraphidium arcuatum (Korsch.) Hind. in the range of concentrations from 0.0001 to 1 mg/L. The toxicity of colloidal silver was manifested at a concentration of 0.1 mg/L and higher and was associated with an algostatic effect, which duration directly depended on silver concentration in the medium. After a period of growth suppression at a concentration of 0.1 mg/L, in both species cell population in the culture was gradually recovered and the structure of population (ratio between the fractions of dividing, quiescent, and dead cells) became the same as in control material. The effect of adaptation of S. quadricauda to colloidal silver was detected upon successive passages to the media with toxic concentrations (0.1 and 0.5 mg/L), which depended on the concentration and duration of initial intoxication of the culture. The toxicity of cell-free filtrates from the culture of S. quadricauda, which was in contact with colloidal silver for different time periods, was estimated using intact culture of the same species, whose population on cell-free filtrates was close to control level. Transfer of the cells that were exposed to silver to a pure medium led to a long lag phase in all the cases. It is assumed that the main mechanism of algal population adaptation to higher concentrations (0.1 and 0.5 mg/L) of this toxicant depends on the selection of resistant cells present in the initial heterogeneous population in the course of primary exposure to colloidal silver. These resistant cells accidentally emerge in the population by means of rare spontaneous mutations occurring in the course of replication even before the contact with silver and have more perfect mechanisms of its detoxification.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Feb 15, 2015

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off