Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of Caenorhabditis elegans

Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of... This study investigated the neurological effects of residual ground-water levels of thiacloprid on the non-target organism Caenorhabditis elegans. Nematodes treated with thiacloprid showed a dose-dependent and significantly increased twitch response at concentrations above 50 ng mL−1 that disabled their forward locomotion in liquid culture. In comparison with untreated controls, 10 ng mL−1 thiacloprid perturbed the chemosensory ability of C. elegans such that the nematodes no longer demonstrated positive chemotaxis towards a NaCl chemo-attractant, reducing their chemotaxis index from +0.48 to near to zero. Nematodes also exhibited a locomotion characteristic of those devoid of chemo-attraction, making significantly more pirouetting turns of ≥90° than the untreated controls. Compared to the untreated controls, expression of the endocytosis-associated gene, Rab-10, was also increased in C. elegans that had developed to adulthood in the presence of 10 ng mL−1 thiacloprid, suggesting their active engagement in increased recycling of affected cellular components, such as their nAChRs. Thus, even residual, low levels of this less potent neonicotinoid that may be found in field ground-water had measurable effects on a beneficial soil organism which may have environmental and ecological implications that are currently poorly understood. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Ecotoxicology Springer Journals

Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of Caenorhabditis elegans

Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of Caenorhabditis elegans

Ecotoxicology (2017) 26:981–990 DOI 10.1007/s10646-017-1826-z Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of Caenorhabditis elegans 1 1 1 1 ● ● ● ● Hannah Hopewell Kieran G. Floyd Daniel Burnell John T. Hancock 1 1 1 ● ● Joel Allainguillaume Michael R. Ladomery Ian D. Wilson Accepted: 10 June 2017 / Published online: 22 June 2017 © The Author(s) 2017. This article is an open access publication Abstract This study investigated the neurological effects Introduction of residual ground-water levels of thiacloprid on the non- target organism Caenorhabditis elegans. Nematodes treated Thiacloprid is a member of the neonicotinoid family of with thiacloprid showed a dose-dependent and significantly systemic insecticides which act as agonists of the post- increased twitch response at concentrations above 50 ng synaptic ligand operated ion channels of the nervous sys- −1 mL that disabled their forward locomotion in liquid cul- tem, the nicotinic acetylcholine receptors (nAChRs) −1 ture. In comparison with untreated controls, 10 ng mL (Matsuda et al. 2005, 2009). Irreversible binding of neoni- thiacloprid perturbed the chemosensory ability of C. ele- cotinoids to nAChRs induces muscular over-stimulation, gans such that the nematodes no longer demonstrated paralysis and death (Yamamoto 1999; Vo et al. 2010; positive chemotaxis towards a NaCl chemo-attractant, Easton and Goulson 2013). Unlike nicotine, the neonicoti- reducing their chemotaxis index from +0.48 to near to zero. noids are characterised by a common pharmacophore con- Nematodes also exhibited a locomotion characteristic of taining either a nitroimine, nitromethylene or cyanoimine those devoid of chemo-attraction, making significantly group which confers, supposedly, selective toxicity because more pirouetting turns of ≥90° than the untreated controls. the specific amino acids of the loop structures of the ligand Compared to the...
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Publisher
Springer US
Copyright
Copyright © 2017 by The Author(s)
Subject
Environment; Environment, general; Ecotoxicology; Ecology; Environmental Management
ISSN
0963-9292
eISSN
1573-3017
D.O.I.
10.1007/s10646-017-1826-z
Publisher site
See Article on Publisher Site

Abstract

This study investigated the neurological effects of residual ground-water levels of thiacloprid on the non-target organism Caenorhabditis elegans. Nematodes treated with thiacloprid showed a dose-dependent and significantly increased twitch response at concentrations above 50 ng mL−1 that disabled their forward locomotion in liquid culture. In comparison with untreated controls, 10 ng mL−1 thiacloprid perturbed the chemosensory ability of C. elegans such that the nematodes no longer demonstrated positive chemotaxis towards a NaCl chemo-attractant, reducing their chemotaxis index from +0.48 to near to zero. Nematodes also exhibited a locomotion characteristic of those devoid of chemo-attraction, making significantly more pirouetting turns of ≥90° than the untreated controls. Compared to the untreated controls, expression of the endocytosis-associated gene, Rab-10, was also increased in C. elegans that had developed to adulthood in the presence of 10 ng mL−1 thiacloprid, suggesting their active engagement in increased recycling of affected cellular components, such as their nAChRs. Thus, even residual, low levels of this less potent neonicotinoid that may be found in field ground-water had measurable effects on a beneficial soil organism which may have environmental and ecological implications that are currently poorly understood.

Journal

EcotoxicologySpringer Journals

Published: Jun 22, 2017

References

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