Replication of Chinese sacbrood virus in primary cell cultures of Asian honeybee ( Apis cerana )

Replication of Chinese sacbrood virus in primary cell cultures of Asian honeybee ( Apis cerana ) A primary cell culture system was established for the first time from embryonic tissues of Asian honeybee, Apis cerana , and used to trace the early infection process of Chinese sacbrood virus (CSBV), an iflavirus in the family Iflaviridae . A monolayer of epithelium-like cells of A. cerana , approximately 8–10 μm in diameter, was grown in Kimura’s insect medium at 28 °C within 3–4 days of setting up the cultures. Such cultured cells were inoculated with CSBV purified from infected larvae or pupae for 2 h. In electron and confocal micrographs, viral particles accumulated as filamentous or vesicular inclusions in the cytoplasm of infected cultured cells at 36 h post-inoculation (hpi). Real-time quantitative RT-PCR assay showed that the expression levels of four cistrons of CSBV in the cultured cells increased rapidly between 12 and 48 hpi. This newly established primary cell culture derived from A. cerana will be useful for further studies of infection caused by CSBV. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Replication of Chinese sacbrood virus in primary cell cultures of Asian honeybee ( Apis cerana )

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Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2183-3
Publisher site
See Article on Publisher Site

Abstract

A primary cell culture system was established for the first time from embryonic tissues of Asian honeybee, Apis cerana , and used to trace the early infection process of Chinese sacbrood virus (CSBV), an iflavirus in the family Iflaviridae . A monolayer of epithelium-like cells of A. cerana , approximately 8–10 μm in diameter, was grown in Kimura’s insect medium at 28 °C within 3–4 days of setting up the cultures. Such cultured cells were inoculated with CSBV purified from infected larvae or pupae for 2 h. In electron and confocal micrographs, viral particles accumulated as filamentous or vesicular inclusions in the cytoplasm of infected cultured cells at 36 h post-inoculation (hpi). Real-time quantitative RT-PCR assay showed that the expression levels of four cistrons of CSBV in the cultured cells increased rapidly between 12 and 48 hpi. This newly established primary cell culture derived from A. cerana will be useful for further studies of infection caused by CSBV.

Journal

Archives of VirologySpringer Journals

Published: Dec 1, 2014

References

  • Genetic and phylogenetic analysis of South Korean sacbrood virus isolates from infected honey bees ( Apis cerana )
    Choe, SE; Nguyen, TT; Hyun, BH; Noh, JH; Lee, HS; Lee, CH; Kang, SW

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