Replication of Chinese sacbrood virus in primary cell cultures of Asian honeybee ( Apis cerana )

Replication of Chinese sacbrood virus in primary cell cultures of Asian honeybee ( Apis cerana ) A primary cell culture system was established for the first time from embryonic tissues of Asian honeybee, Apis cerana , and used to trace the early infection process of Chinese sacbrood virus (CSBV), an iflavirus in the family Iflaviridae . A monolayer of epithelium-like cells of A. cerana , approximately 8–10 μm in diameter, was grown in Kimura’s insect medium at 28 °C within 3–4 days of setting up the cultures. Such cultured cells were inoculated with CSBV purified from infected larvae or pupae for 2 h. In electron and confocal micrographs, viral particles accumulated as filamentous or vesicular inclusions in the cytoplasm of infected cultured cells at 36 h post-inoculation (hpi). Real-time quantitative RT-PCR assay showed that the expression levels of four cistrons of CSBV in the cultured cells increased rapidly between 12 and 48 hpi. This newly established primary cell culture derived from A. cerana will be useful for further studies of infection caused by CSBV. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Replication of Chinese sacbrood virus in primary cell cultures of Asian honeybee ( Apis cerana )

Loading next page...
 
/lp/springer_journal/replication-of-chinese-sacbrood-virus-in-primary-cell-cultures-of-w9DLOO6mHM
Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2183-3
Publisher site
See Article on Publisher Site

Abstract

A primary cell culture system was established for the first time from embryonic tissues of Asian honeybee, Apis cerana , and used to trace the early infection process of Chinese sacbrood virus (CSBV), an iflavirus in the family Iflaviridae . A monolayer of epithelium-like cells of A. cerana , approximately 8–10 μm in diameter, was grown in Kimura’s insect medium at 28 °C within 3–4 days of setting up the cultures. Such cultured cells were inoculated with CSBV purified from infected larvae or pupae for 2 h. In electron and confocal micrographs, viral particles accumulated as filamentous or vesicular inclusions in the cytoplasm of infected cultured cells at 36 h post-inoculation (hpi). Real-time quantitative RT-PCR assay showed that the expression levels of four cistrons of CSBV in the cultured cells increased rapidly between 12 and 48 hpi. This newly established primary cell culture derived from A. cerana will be useful for further studies of infection caused by CSBV.

Journal

Archives of VirologySpringer Journals

Published: Dec 1, 2014

References

  • Genetic and phylogenetic analysis of South Korean sacbrood virus isolates from infected honey bees ( Apis cerana )
    Choe, SE; Nguyen, TT; Hyun, BH; Noh, JH; Lee, HS; Lee, CH; Kang, SW

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from
Google Scholar,
PubMed
Create lists to
organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off