Replication of a chronic hepatitis B virus genotype F1b construct

Replication of a chronic hepatitis B virus genotype F1b construct Genotype F is one of the less-studied genotypes of human hepatitis B virus, although it is widely distributed in regions of Central and South American. Our previous studies have shown that HBV genotype F is prevalent in Chile, and phylogenetic analysis of its full-length sequence amplified from the sera of chronically infected patients identified it as HBV subgenotype F1b. We have previously reported the full-length sequence of a HBV molecular clone obtained from a patient chronically infected with genotype F1b. In this report, we established a system to study HBV replication based on hepatoma cell lines transfected with full-length monomers of the HBV genome. Culture supernatants were analyzed after transfection and found to contain both HBsAg and HBeAg viral antigens. Consistently, fractionated cell extracts revealed the presence of viral replication, with both cytoplasmic and nuclear DNA intermediates. Analysis of HBV-transfected cells by indirect immunofluorescence or immunoelectron microscopy revealed the expression of viral antigens and cytoplasmic viral particles, respectively. To test the functionality of the ongoing viral replication further at the level of chromatinized cccDNA, transfected cells were treated with a histone deacetylase inhibitor, and this resulted in increased viral replication. This correlated with changes posttranslational modifications of histones at viral promoters. Thus, the development of this viral replication system for HBV genotype F will facilitate studies on the regulation of viral replication and the identification of new antiviral drugs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

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Publisher
Springer Vienna
Copyright
Copyright © 2016 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2702-x
Publisher site
See Article on Publisher Site

References

  • Hepatitis B virus–cell interactions and pathogenesis
    Nguyen, D; Ludgate, L; Hu, J
  • Structural organization of the hepatitis B virus minichromosome
    Bock, C; Schwinn, S; Locarnini, S; Fyfe, J; Manns, M
  • Hepatitis B virus replication is regulated by the acetylation status of hepatitis B virus cccDNA-Bound H3 and H4 histones
    Pollicino, T; BelloniI, L; Raffa, G; Pediconi, N; Squadrito, G
  • Worldwide genetic diversity of HBV genotypes and risk of hepatocellular carcinoma
    Pujol, F; Navas, M; Hainaut, P; Chemin, I
  • HBV genotype and clinical outcome of chronic hepatitis B: facts and puzzles
    Tseng, T; Kao, J
  • Five subgenotypes of hepatitis B virus genotype B with distinct geographic and virological characteristics
    Liu, W; Phiet, P; Chiang, T; Sun, K; Hung, K
  • The clinical implications of hepatitis B virus genotype: recent advances
    Lin, C; Kao, J
  • Prevalence of hepatitis B virus genotypes in chronic carriers in Santiago, Chile
    Venegas, M; Munoz, G; Hurtado, C; Alvarez, L; Velasco, M
  • Hepatitis B viruses: reverse transcription a different way
    Nassal, M
  • The catalytic properties of human hepatitis B virus polymerase
    Jeong, J; Kwak, D; Rho, H; Jung, G
  • Hepatitis B virus replication in human HepG2 cells mediated by hepatitis B virus recombinant baculovirus
    Delaney, W; Isom, H

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