Access the full text.
Sign up today, get DeepDyve free for 14 days.
K. Williams, Annette Schwartz, S. Corey, M. Orandle, William Kennedy, B. Thompson, X. Alvarez, Charles Brown, S. Gartner, A. Lackner (2002)
Proliferating cellular nuclear antigen expression as a marker of perivascular macrophages in simian immunodeficiency virus encephalitis.The American journal of pathology, 161 2
T. Steinfeldt, T. Finsterbusch, A. Mankertz (2001)
Rep and Rep' protein of porcine circovirus type 1 bind to the origin of replication in vitro.Virology, 291 1
C. Gutiérrez (1999)
Geminivirus DNA replicationCellular and Molecular Life Sciences CMLS, 56
G. Allan, F. Mcneilly, J. Cassidy, G. Reilly, B. Adair, W. Ellis, M. Mcnulty (1995)
Pathogenesis of porcine circovirus; experimental infections of colostrum deprived piglets and examination of pig foetal material.Veterinary microbiology, 44 1
R. Sanchez, P. Meerts, H. Nauwynck, J. Ellis, M. Pensaert (2004)
Characteristics of Porcine Circovirus—2 Replication in Lymphoid Organs of Pigs Inoculated in Late Gestation or Postnatally and Possible Relation to Clinical and Pathological Outcome of InfectionJournal of Veterinary Diagnostic Investigation, 16
R. Sanchez, P. Meerts, H. Nauwynck, M. Pensaert (2003)
Change of porcine circovirus 2 target cells in pigs during development from fetal to early postnatal life.Veterinary microbiology, 95 1-2
K. West, J. Bystrom, C. Wojnarowicz, Neil Shantz, M. Jacobson, G. Allan, D. Haines, E. Clark, S. Krakowka, F. Mcneilly, C. Konoby, K. Martin, J. Ellis (1999)
Myocarditis and Abortion Associated with Intrauterine Infection of Sows with Porcine Circovirus 2Journal of Veterinary Diagnostic Investigation, 11
G. Allan, F. Mcneilly, J. Foster, B. Adair (1994)
Infection of leucocyte cell cultures derived from different species with pig circovirus.Veterinary microbiology, 41 3
I. Tischer, H. Buhk (1988)
Viral DNA from cells infected with porcine circovirus.Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology, 270 1-2
Q. Liu, S. Tikoo, L. Babiuk (2001)
Nuclear localization of the ORF2 protein encoded by porcine circovirus type 2.Virology, 285 1
F. Mcneilly, I. McNair, D. Mackie, B. Meehan, S. Kennedy, Deborah Moffett, J. Ellis, S. Krakowka, G. Allan (2001)
Production, characterisation and applications of monoclonal antibodies to porcine circovirus 2Archives of Virology, 146
Qiang Liu, S. Tikoo, L. Babiuk (2001)
Nuclear Localization of the ORF 2 Protein Encoded by Porcine Circovirus Type 2
A. Mankertz, J. Mankertz, Katarina Wolf, Hans-Joerg Buhk (1998)
Identification of a protein essential for replication of porcine circovirus.The Journal of general virology, 79 ( Pt 2)
H. Kim (1996)
Expression of intermediate filament desmin and vimentin in the human fetal heartThe Anatomical Record, 246
A.-S. Ladekjær-Mikkelsen, J. Nielsen, T. Storgaard, A. Bøtner, G. Allan, F. Mcneilly (2001)
Transplacental infection with PCV-2 associated with reproductive failure in a gilt.The Veterinary record, 148 24
A. Mankertz, Bernd Hillenbrand (2001)
Replication of porcine circovirus type 1 requires two proteins encoded by the viral rep gene.Virology, 279 2
J. Alemán (1988)
Author's address:
D. Rasschaert, M. Duarte, H. Laude (1990)
Porcine respiratory coronavirus differs from transmissible gastroenteritis virus by a few genomic deletions.The Journal of general virology, 71 ( Pt 11)
P. Nawagitgul, Igor Morozov, S. Bolin, P. Harms, S. Sorden, P. Paul (2000)
Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein.The Journal of general virology, 81 Pt 9
N. Vanderheijden, P. Delputte, H. Favoreel, J. Vandekerckhove, J. Damme, P. Woensel, H. Nauwynck (2003)
Involvement of Sialoadhesin in Entry of Porcine Reproductive and Respiratory Syndrome Virus into Porcine Alveolar MacrophagesJournal of Virology, 77
I. Tischer, D. Peters, R. Rasch, S. Pociuli (2005)
Replication of porcine circovirus: induction by glucosamine and cell cycle dependenceArchives of Virology, 96
J. Donson, B. Morris-Krsinich, P. Mullineaux, M. Boulton, J. Davies (1984)
A putative primer for second‐strand DNA synthesis of maize streak virus is virion‐associated.The EMBO Journal, 3
F. Blecha, T. Kielian, D. McVey, J. Lunney, K. Walker, C. Stokes, K. Stevens, Y. Kim, R. Chu, T. Chen (1994)
Workshop studies on monoclonal antibodies reactive against porcine myeloid cells.Veterinary immunology and immunopathology, 43 1-3
A. Cheung, S. Bolin (2002)
Kinetics of porcine circovirus type 2 replicationArchives of Virology, 147
B. Meehan, F. Mcneilly, I. McNair, I. Walker, J. Ellis, S. Krakowka, G. Allan (2001)
Isolation and characterization of porcine circovirus 2 from cases of sow abortion and porcine dermatitis and nephropathy syndromeArchives of Virology, 146
P. Meerts, H. Nauwynck, R. Sanchez, B. Mateusen, M. Pensaert (2004)
Prevalence of porcine circovirus 2 (pcv2)-related wasting on belgian farms with or without a history of postweaning multisystemic wasting syndromeVlaams Diergeneeskundig Tijdschrift
I. Tischer, W. Mields, D. Wolff, M. Vagt, W. Griem (2005)
Studies on epidemiology and pathogenicity of porcine circovirusArchives of Virology, 91
J. Ellis, L. Hassard, E. Clark, J. Harding, G. Allan, Philip Willson, John Strokappe, K. Martin, F. Mcneilly, B. Meehan, D. Todd, D. Haines (1998)
Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome.The Canadian veterinary journal = La revue veterinaire canadienne, 39 1
R. Sanchez, H. Nauwynck, F. Mcneilly, G. Allan, M. Pensaert (2001)
Porcine circovirus 2 infection in swine foetuses inoculated at different stages of gestation.Veterinary microbiology, 83 2
D. Gilpin, K. McCullough, B. Meehan, F. Mcneilly, I. McNair, L. Stevenson, J. Foster, J. Ellis, S. Krakowka, B. Adair, G. Allan (2003)
In vitro studies on the infection and replication of porcine circovirus type 2 in cells of the porcine immune system.Veterinary immunology and immunopathology, 94 3-4
G. Labarque, Hans Nauwynck, K. Reeth, M. Pensaert (2000)
Effect of cellular changes and onset of humoral immunity on the replication of porcine reproductive and respiratory syndrome virus in the lungs of pigs.The Journal of general virology, 81 Pt 5
G. Allan, J. Ellis (2000)
Porcine Circoviruses: A ReviewJournal of Veterinary Diagnostic Investigation, 12
I. Tischer, H. Gelderblom, W. Vettermann, M. Koch (1982)
A very small porcine virus with circular single-stranded DNANature, 295
In this in vitro study, the replication kinetics of porcine circovirus type 2 (PCV2) in porcine alveolar macrophages (PAM) and fetal cardiomyocytes (FCM), two target cells in vivo , was compared with that in PK-15 cells. Cultures were inoculated with either the postweaning multisystemic wasting syndrome (PMWS)-associated strain Stoon-1010 or the abortion-associated strain 1121. Viral proteins were visualized and virus production was determined. In PK-15 cells, the capsid protein was expressed between 6 and 12 hours post inoculation (hpi), it relocated to the nucleus between 12 and 24 hpi. At that time, Rep protein was also detected in the nucleus. This sequence of events also occurred in FCM and PAM but nuclear localized antigens appeared later (48 hpi) and in a lower percentage of cells. In PAM, clear differences in susceptibility were seen between pigs. In PAM from two out of five tested pigs, nuclear localized antigens were not detected, whereas in PAM from three other pigs they were seen in up to 20% of the antigen-positive cells. Virus production was observed in PK-15 but not in PAM or FCM cultures. In a second study, the replication kinetics of seven different PCV2 strains were compared in PK-15 cells. It was shown that the two abortion-associated strains had a different replication kinetics in comparison with PMWS or porcine dermatitis and nephropathy syndrome associated strains. With the abortion-associated strains, a higher number of infected cells was observed at 24 hpi and the percentage of infected cells with nuclear localised antigens was lower compared to that of other strains.
Archives of Virology – Springer Journals
Published: Mar 1, 2005
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.