1070-4272/02/7503-0509$27.00C2002 MAIK [Nauka/Interperiodica]
Russian Journal of Applied Chemistry, Vol. 75, No. 3, 2002, pp. 509!510. Translated from Zhurnal Prikladnoi Khimii, Vol. 75, No. 3,
2002, pp. 520!521.
Original Russian Text Copyright + 2002 by Komarova, Sul’man.
Relationships of Sorption of Heavy Metals (Lead, Nickel,
Cobalt) on Biomass Isolated from Production Waste
of Megaterin Enzyme Preparation
N. N. Komarova and E. M. Sul’man
Tver State Technical University, Tver, Russia
Received April 18, 2001
Abstract-The biomass of Bacillus megaterium cells, containing polypeptides, polysaccharides, and lipids,
isolated from production waste of Megaterin enzyme preparation, was tested for sorption recovery of heavy
metals (lead, nickel, cobalt) from aqueous solutions. The sorption capacity of this biosorbent and the main
relationships of the metal sorption were studied.
Modern processes for water decontamination from
various impurities tend to wider use of sorbents
prepared from bacterial waste of biotechnological
productions . The microbial biomass used as a raw
material for preparation of biosorbents is formed
in the course of microbial synthesis. This material
consists of partly destroyed cells of microorganisms
containing biopolymers rich in ionogenic groups .
It is known that heavy metal ions are toxic agents.
At the same time, heavy metals incorporated in com-
plexes lose toxicity. The biomass of microbial cells
can be effective natural sorbent accumulating heavy
metals on the cell surface. The aim of this work was
to study the sorption capability of microbial biomass
for heavy metals.
The microbial biomass used in our experiments
(Bacillus megaterium) was isolated from production
waste of Megaterin enzyme preparation.
power of bacterial cells with respect to heavy metals
and the optimal conditions for metal sorption were
studied using aqueous solutions of cobalt chloride
, nickel acetate Ni(CH
, and lead acetate
. The sample of dry biomass taken
from the enzyme-producing plant was treated with
acetone. Excess acetone was removed by vacuum fil-
tration. Prior to use, the biomass was washed with
distilled water to neutral reaction of wash water and
dried [1, 2]. A portion of the resulting biomass was
The material was produced and submitted by the Vyshni
Volochek plant of Enzymatic Preparations.
introduced into a model solution containing heavy
metal ions. The sorption was carried out in a tempera-
ture-controlled reactor at stirring. After completion of
a sorption experiment, the biomass was separated by
centrifuging. The residual content of Co(II), Ni(II),
and Pb(II) in the cenrifuged solution was determined
spectrophotometrically and fluorometrically .
We found that at a constant metal concentration
(0.1 g l
) in solution and a constant solution volume
(250 ml) the residual metal content in solution con-
tinuously decreased with increasing sorbent weight.
The results obtained in the sorption experiments
showed that the sorption was maximal at the sorbent
concentration of 80390 g l
The sorption isotherm has a convex shape and is
fairly well fitted by Langmuir’s equation (Fig. 1). The
metal ion concentration in the solution was varied
from 0.01 to 0.2 g l
. Sorption was performed to
Fig. 1. Sorption isotherms of metals on microbial biomass:
(S) sorption capacity and (C) equilibrium metal concentra-
tion in solution. Cation sorbed: (1)Pb
: the same for Fig. 2.