Arch Virol (1998) 143: 1823–1829
Relationship of the pelargonium ﬂower break carmovirus (PFBV)
coat protein gene with that of other carmoviruses
, C. Kusiak
, J. P. Renou
, J. Albouy
,and S. Dinant
INRA, Laboratoire de Biologie Cellulaire, Versailles, France
INRA, Station d’Amélioration des Espéces Fruitiéres et Ornementales, Angers, France
INRA, Station de Pathologie Végétale, Versailles, France
Accepted April 23, 1998
-terminal1500 nucleotidesof thegenome ofpelargoniumﬂower
break carmovirus (PFBV) were sequenced from RT-PCR ampliﬁcation products.
One large ORF was found, encoding a 345 amino acid protein of Mr 37 kDa,
which corresponds to the coat protein, as conﬁrmed by immunoprecipitation of
products of in vitro transcription and translation. The sequence also included the
putative promoter of the coat protein gene subgenomic RNA, as well as its 5
untranslated regions. The PFBV coat protein was more similar to that of
saguaro cactus virus and carnation mottle virus than to that of other carmoviruses.
Despite the lower level of similarity of CP gene sequences compared to the RNA
dependent RNA polymerase (RdRp) gene sequences of small icosahedral viruses
used in taxonomic studies, PFBV CP sequence comparisons and alignments con-
ﬁrmed that PFBV is related to carmoviruses, tombusviruses and a dianthovirus,
as previously concluded from the analysis of a PFBV RdRp gene fragment.
Pelargoniumﬂower break carmovirus (PFBV) is the most common viral pathogen
on Pelargonium species, causing characteristic ﬂower discolorations and reduced
vegetative growth on cultivated pelargoniums that result in a decrease in plant
quality . The primary means of transmission of PFBV in western Europe is
by vegetative propagation, since pelargonium plants are mainly produced from
cuttings. PFBV is also transmitted mechanically, by irrigation systems, or by
virus-infected pollen transported by the thrips Frankiniella occidentalis .
The EMBL accession number of the sequence reported in this paper is AJ003153