Regulation of the Epstein-Barr viral immediate early BRLF1 promoter through a distal NF1 site

Regulation of the Epstein-Barr viral immediate early BRLF1 promoter through a distal NF1 site The immediate early BRLF1 and BZLF1 promoters of Epstein-Barr virus are crucial for triggering the replicative cycle of the virus. To better understand the cell type dependence of the lytic cycle we conducted an analysis of the BRLF1-promoter in the epithelial cell line HeLa and the lymphoid cell line IM9. To analyze promoter activities, transient transfections with 5′-deletions of the BRLF1-promoter in front of luciferase as reporter gene were conducted. Besides the already known cis -acting elements of the promoter close to the TATA-box, more distal elements were located and functionally tested. A nuclear factor 1 consensus site was found to act positively in HeLa cells, but did not in lymphoid IM9 cells. The NF1 site was shown to bind protein by electrophoretic mobility shift assays, antibody-supershifts and in vitro footprinting. Thus, a protein belonging to the nuclear factor 1 family of proteins was identified as additional cellular trans -acting factor for the BRLF1-promoter besides the already described factors Sp1, Zta and Zif268. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Regulation of the Epstein-Barr viral immediate early BRLF1 promoter through a distal NF1 site

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Publisher
Springer-Verlag
Copyright
Copyright © Wien by 1998 Springer-Verlag/
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050050433
Publisher site
See Article on Publisher Site

Abstract

The immediate early BRLF1 and BZLF1 promoters of Epstein-Barr virus are crucial for triggering the replicative cycle of the virus. To better understand the cell type dependence of the lytic cycle we conducted an analysis of the BRLF1-promoter in the epithelial cell line HeLa and the lymphoid cell line IM9. To analyze promoter activities, transient transfections with 5′-deletions of the BRLF1-promoter in front of luciferase as reporter gene were conducted. Besides the already known cis -acting elements of the promoter close to the TATA-box, more distal elements were located and functionally tested. A nuclear factor 1 consensus site was found to act positively in HeLa cells, but did not in lymphoid IM9 cells. The NF1 site was shown to bind protein by electrophoretic mobility shift assays, antibody-supershifts and in vitro footprinting. Thus, a protein belonging to the nuclear factor 1 family of proteins was identified as additional cellular trans -acting factor for the BRLF1-promoter besides the already described factors Sp1, Zta and Zif268.

Journal

Archives of VirologySpringer Journals

Published: Oct 1, 1998

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