Regulation of in vitro and in vivo differentiation of mouse embryonic stem cells, embryonic germ cells and teratocarcinoma cells by TGFβ family signaling factors

Regulation of in vitro and in vivo differentiation of mouse embryonic stem cells, embryonic germ... The activity of specific signaling and transcription factors determines the cell fate in normal development and in tumor transformation. The transcriptional profiles of gene-components of different branches of TGFβ family signaling pathways were studied in experimental models of initial stages of three-dimensional in vitro differentiation of embryonic stem cells, embryonic germ cells and teratocarcinoma cells and in teratomas and teratocarcinomas developed after their transplantation into immunodeficient Nude mice. Gene profile analysis of studied cell systems have revealed that expression patterns of ActivinA, Nodal, Lefty1, Lefty2, Tgfβ1, Bmp4, and GDF3 were identical in pluripotent stem cells whereas the mRNAs of all examined genes with the exception of (Inhibin Inhibin β A /ActivinA) were detected in the teratocarcinoma cells. These results indicate that differential activity of signaling pathways of the TGFβ family factors regulates the pluripotent state maintenance and pluripotent stem cell differentiation into the progenitors of three germ layers and extraembryonic structures and that normal expression pattern of TGFβ family factors is rearranged in embryonic teratocarcinoma cells during tumor growth in vitro and in vivo. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Developmental Biology Springer Journals

Regulation of in vitro and in vivo differentiation of mouse embryonic stem cells, embryonic germ cells and teratocarcinoma cells by TGFβ family signaling factors

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2009 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Animal Anatomy / Morphology / Histology; Developmental Biology
ISSN
1062-3604
eISSN
1608-3326
D.O.I.
10.1134/S1062360409060010
Publisher site
See Article on Publisher Site

Abstract

The activity of specific signaling and transcription factors determines the cell fate in normal development and in tumor transformation. The transcriptional profiles of gene-components of different branches of TGFβ family signaling pathways were studied in experimental models of initial stages of three-dimensional in vitro differentiation of embryonic stem cells, embryonic germ cells and teratocarcinoma cells and in teratomas and teratocarcinomas developed after their transplantation into immunodeficient Nude mice. Gene profile analysis of studied cell systems have revealed that expression patterns of ActivinA, Nodal, Lefty1, Lefty2, Tgfβ1, Bmp4, and GDF3 were identical in pluripotent stem cells whereas the mRNAs of all examined genes with the exception of (Inhibin Inhibin β A /ActivinA) were detected in the teratocarcinoma cells. These results indicate that differential activity of signaling pathways of the TGFβ family factors regulates the pluripotent state maintenance and pluripotent stem cell differentiation into the progenitors of three germ layers and extraembryonic structures and that normal expression pattern of TGFβ family factors is rearranged in embryonic teratocarcinoma cells during tumor growth in vitro and in vivo.

Journal

Russian Journal of Developmental BiologySpringer Journals

Published: Dec 3, 2009

References

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