Regulation of endoreduplication in maize (Zea mays L.) endosperm. Isolation of a novel B1-type cyclin and its quantitative analysis

Regulation of endoreduplication in maize (Zea mays L.) endosperm. Isolation of a novel B1-type... To investigate the involvement of cyclin in mitotic and endoreduplicative cell cycle control, we have isolated a mitotic cyclin clone from a maize endosperm cDNA library. The deduced amino acid sequence of this clone identifies a novel B1-type cyclin with distinctly different sequence in regions with putative involvement in intracellular localization. This cyclin, designated Zeama;CycB1;3 (CycZme1), was shown by RNA gel blots and quantitative RT-PCR to be specific for tissues engaging in cell proliferation. It accumulated in metaphase-arrested cells and declined rapidly upon release into G1 phase. During the transition from mitosis to endoreduplication in maize endosperm, CycZme1 transcript declined precipitously while transcripts associated with S phase (histone-H3 and PCNA) and multiple phases of the cell cycle (Cdc2, α-tubulin) remained at moderate to high levels. We conclude that CycZme1 down-regulation is involved in the cellular transition to endoreduplication. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Regulation of endoreduplication in maize (Zea mays L.) endosperm. Isolation of a novel B1-type cyclin and its quantitative analysis

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 1999 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1006315625486
Publisher site
See Article on Publisher Site

Abstract

To investigate the involvement of cyclin in mitotic and endoreduplicative cell cycle control, we have isolated a mitotic cyclin clone from a maize endosperm cDNA library. The deduced amino acid sequence of this clone identifies a novel B1-type cyclin with distinctly different sequence in regions with putative involvement in intracellular localization. This cyclin, designated Zeama;CycB1;3 (CycZme1), was shown by RNA gel blots and quantitative RT-PCR to be specific for tissues engaging in cell proliferation. It accumulated in metaphase-arrested cells and declined rapidly upon release into G1 phase. During the transition from mitosis to endoreduplication in maize endosperm, CycZme1 transcript declined precipitously while transcripts associated with S phase (histone-H3 and PCNA) and multiple phases of the cell cycle (Cdc2, α-tubulin) remained at moderate to high levels. We conclude that CycZme1 down-regulation is involved in the cellular transition to endoreduplication.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 16, 2004

References

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