Russian Journal of Applied Chemistry, 2009, Vol. 82, No. 5, pp. 794−800.
Pleiades Publishing, Ltd., 2009.
Original Russian Text
L.A. Deineka, A.N. Chulkov, I.I. Saenko, V.I. Deineka, 2009, published in Zhurnal Prikladnoi Khimii, 2009, Vol. 82, No. 5,
OF SYSTEMS AND PROCESSES
Regularities in Adsorption of Anthocyans
by Natural Clays
L. A. Deineka, A. N. Chulkov, I. I. Saenko, and V. I. Deineka,
Belgorod State University, Belgorod, Russia
Received January 29, 2008
Abstract—Regularities of adsorption by clay of some commercial sorts of anthocyans extracted from plant
materials are investigated. The interrelation between sorption ability and structure of sorbates is revealed.
Introduction of organic solvent is shown providing a possibility of targeted changing sorption properties of system
and sorption purification of anthocyans.
Anthocyans are organic substances with the basic
structure in the form of cation; the anthocyans are
valuable colorants for the food and medical industry,
whose important property is a wide spectrum of
biological activity. However, during their extraction
from plant material simultaneously are extracted in a
signiﬁ cant amount some associated substances, whose
nature depends on the source material and the method
of extraction. Therefore puriﬁ cation of anthocyanate-
containing extracts is vital problem.
In this communication is considered concentration
and purification of anthocyans with natural clays.
About the high sorption activity of some commercial
accessible clays has been reported earlier [1–3]. It was
established that the sorption activity of clays depends on
the structure of anthocyans, mainly not of the aglycone
part, but in essence on the structure of carbohydrate
moiety. Use of talc, another laminar inorganic mineral,
for concentration and puriﬁ cation of the grape anthocyans
was described , whereas in the foreign literature was
described application for these purposes of substantially
more expensive synthetic sorbents on the basis of
styrene–divinylbenzene copolymers (SDVB) [5–7]. In
 was described investigation of 11 sorbents for the
concentration of anthocyan from the Hungarian red wine,
therewith the octadecylsilane sorbents showed the highest
sorption capacity; the silica gels modiﬁ ed by aminopropyl
groups was inferior to them, while not modiﬁ ed silica
gel and alumogel were proved to be ineffective for the
There is little information on the application of clays
in the technological stages with the participation of
anthocyans. It is known the use of bentonite for treating
wines for the purpose of increase their stability on the
storage , and in  was investigated adsorption of
anthocyans on the clay with the subsequent desorption
as a method of puriﬁ cation of industrial extracts from the
red radish. It is curious also the method of puriﬁ cation
disclosed in the patent , which includes transformation
of anthocyans into the not colored hydrosulﬁ te complexes.
These complexes are sorbed in the dynamic regime on
macroporous nonionogenic styrene, divinylbenzene
sorbents, with the subsequent reextraction of anthocyans
with acidiﬁ ed alcohol.
Purpose of this work is development of the technology
for the concentration puriﬁ cation of anthocyans with the
use of clay materials.
The anthocyans were extracted from the plant material
by infusion in 0.1 M solution of hydrochloric acid. Extract
was separated from the source material by ﬁ ltration
through a paper ﬁ lter. The adsorption of anthocyans was
determined by the static method: to a measured volume
of extract was added the necessary quantity of clay and