Recovery of avirulent, thermostable Newcastle disease virus strain NDV4-C from cloned cDNA and stable expression of an inserted foreign gene

Recovery of avirulent, thermostable Newcastle disease virus strain NDV4-C from cloned cDNA and... A reverse genetics system for thermostable Newcastle disease virus (NDV) is not currently available. In this study, we developed a reverse genetics system for the avirulent and thermostable NDV4-C strain. Successful recovery of NDV4-C was achieved by using either T7 RNA polymerase or cellular RNA polymerase II to drive transcription of the full-length virus antigenome from cloned cDNA. The recovered viruses rNDV4-C (T7) and rNDV4-C (CMV) showed similar growth properties, thermostability, and virulence as the parental strain NDV4-C. The potential of rNDV4-C (T7) to serve as a viral vector was assessed by generating a recombinant virus, rNDV4-eGFP, which expressed enhanced green fluorescent protein. The rNDV4-eGFP could stably carry and express eGFP for at least fifteen passages. The reverse genetics system for NDV4-C will make it possible to analyze the genetic elements that determine thermostability and the oncolytic properties of NDV. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Recovery of avirulent, thermostable Newcastle disease virus strain NDV4-C from cloned cDNA and stable expression of an inserted foreign gene

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Publisher
Springer Vienna
Copyright
Copyright © 2013 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1723-6
Publisher site
See Article on Publisher Site

Abstract

A reverse genetics system for thermostable Newcastle disease virus (NDV) is not currently available. In this study, we developed a reverse genetics system for the avirulent and thermostable NDV4-C strain. Successful recovery of NDV4-C was achieved by using either T7 RNA polymerase or cellular RNA polymerase II to drive transcription of the full-length virus antigenome from cloned cDNA. The recovered viruses rNDV4-C (T7) and rNDV4-C (CMV) showed similar growth properties, thermostability, and virulence as the parental strain NDV4-C. The potential of rNDV4-C (T7) to serve as a viral vector was assessed by generating a recombinant virus, rNDV4-eGFP, which expressed enhanced green fluorescent protein. The rNDV4-eGFP could stably carry and express eGFP for at least fifteen passages. The reverse genetics system for NDV4-C will make it possible to analyze the genetic elements that determine thermostability and the oncolytic properties of NDV.

Journal

Archives of VirologySpringer Journals

Published: Oct 1, 2013

References

  • Third genome size category of avian paramyxovirus serotype 1 (Newcastle disease virus) and evolutionary implications
    Czegledi, A; Ujvari, D; Somogyi, E; Wehmann, E; Werner, O; Lomniczi, B
  • An assessment of the Australian V4 strain of Newcastle disease virus as a vaccine by spray, aerosol and drinking water administration
    Ibrahim, AL; Chulan, U; Babjee, AM
  • Genome sequence of the thermostable Newcastle disease virus (strain I-2) reveals a possible phenotypic locus
    Kattenbelt, JA; Meers, J; Gould, AR
  • Administration of a vaccine prepared from the Australian V4 strain of Newcastle disease virus by aerosol and drinking water
    Kim, SJ; Spradbrow, PB
  • Genome replication of Newcastle disease virus: involvement of the rule-of-six
    Peeters, BP; Gruijthuijsen, YK; Leeuw, OS; Gielkens, AL

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