Recombinant infectious bronchitis virus (IBV) H120 vaccine strain expressing the hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) protects chickens against IBV and NDV challenge

Recombinant infectious bronchitis virus (IBV) H120 vaccine strain expressing the... Infectious bronchitis (IB) and Newcastle disease (ND) are common viral diseases of chickens, which are caused by infectious bronchitis virus (IBV) and Newcastle disease virus (NDV), respectively. Vaccination with live attenuated strains of IBV-H120 and NDV-LaSota are important for the control of IB and ND. However, conventional live attenuated vaccines are expensive and result in the inability to differentiate between infected and vaccinated chickens. Therefore, there is an urgent need to develop new efficacious vaccines. In this study, using a previously established reverse genetics system, we generated a recombinant IBV virus based on the IBV H120 vaccine strain expressing the haemagglutinin-neuraminidase (HN) protein of NDV. The recombinant virus, R-H120-HN/5a, exhibited growth dynamics, pathogenicity and viral titers that were similar to those of the parental IBV H120, but it had acquired hemagglutination activity from NDV. Vaccination of SPF chickens with the R-H120-HN/5a virus induced a humoral response at a level comparable to that of the LaSota/H120 commercial bivalent vaccine and provided significant protection against challenge with virulent IBV and NDV. In summary, the results of this study indicate that the IBV H120 strain could serve as an effective tool for designing vaccines against IB and other infectious diseases, and the generation of IBV R-H120-HN/5a provides a solid foundation for the development of an effective bivalent vaccine against IBV and NDV. Archives of Virology Springer Journals

Recombinant infectious bronchitis virus (IBV) H120 vaccine strain expressing the hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) protects chickens against IBV and NDV challenge

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Springer Vienna
Copyright © 2016 by Springer-Verlag Wien
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
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  • Coronavirus avian infectious bronchitis virus
    Cavanagh, D
  • Complete genome sequence and recombination analysis of infectious bronchitis virus attenuated vaccine strain H120
    Zhang, Y; Wang, HN; Wang, T; Fan, WQ; Zhang, AY; Wei, K; Tian, GB; Yang, X
  • Origin and characteristics of the recombinant novel avian infectious bronchitis coronavirus isolate ck/CH/LJL/111054
    Liu, S; Xu, Q; Han, Z; Liu, X; Li, H; Guo, H
  • Newcastle disease virus: current status and our understanding
    Ganar, K; Das, M; Sinha, S; Kumar, S
  • Molecular detection and smoothing spline clustering of the IBV strains detected in China during 2011–2012
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  • Molecular and antigenic characteristics of Massachusetts genotype infectious bronchitis coronavirus in China
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  • Comparative analysis of four Massachusetts type infectious bronchitis coronavirus genomes reveals a novel Massachusetts type strain and evidence of natural recombination in the genome
    Liu, X; Shao, Y; Ma, H; Sun, C; Zhang, X; Li, C; Han, Z; Yan, B; Kong, X; Liu, S

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