Reactions of heme proteins with carbonate radical anion

Reactions of heme proteins with carbonate radical anion Reactions of radiolytically generated CO3 •− with some ferric heme proteins, catalase, cytochrome c, and horseradish peroxidase (HRP), were studied. Carbonate radical anion oxidized amino acid residues of these proteins, but did not react directly with heme iron. HRP and catalase lost about 30% and 20% of their activity, respectively, after the reaction with 100 μM of CO3 •−. The rate constants of the reactions of CO3 •− with the investigated proteins measured by the pulse radiolysis method at pH 8–8.4 and 10 varied from 1.0 × 108 M−1 s−1 (for cytochrome c) to 3.7 × 109 M−1 s−1 (for catalase). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Chemical Intermediates Springer Journals

Reactions of heme proteins with carbonate radical anion

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Publisher
Springer Netherlands
Copyright
Copyright © 2009 by Springer Science+Business Media BV
Subject
Chemistry; Inorganic Chemistry ; Physical Chemistry ; Catalysis
ISSN
0922-6168
eISSN
1568-5675
D.O.I.
10.1007/s11164-009-0042-8
Publisher site
See Article on Publisher Site

Abstract

Reactions of radiolytically generated CO3 •− with some ferric heme proteins, catalase, cytochrome c, and horseradish peroxidase (HRP), were studied. Carbonate radical anion oxidized amino acid residues of these proteins, but did not react directly with heme iron. HRP and catalase lost about 30% and 20% of their activity, respectively, after the reaction with 100 μM of CO3 •−. The rate constants of the reactions of CO3 •− with the investigated proteins measured by the pulse radiolysis method at pH 8–8.4 and 10 varied from 1.0 × 108 M−1 s−1 (for cytochrome c) to 3.7 × 109 M−1 s−1 (for catalase).

Journal

Research on Chemical IntermediatesSpringer Journals

Published: Jun 9, 2009

References

  • The carbonate radical and related oxidants derived from bicarbonate buffer
    Medinas, DB; Cerchiaro, G; Trindade, DF; Augusto, O
  • Hydroperoxide metabolism in mammalian organs
    Chance, B; Sies, H; Boveris, A
  • Rate constants for reactions of inorganic radicals in aqueous solution
    Neta, P; Huie, RE; Ross, AB
  • Peroxidase-like activity of cytochrome c in the presence of anionic surfactants
    Gebicka, L
  • Structure of beef liver catalase
    Murthy, MRN; Reid, TJ; Sicignano, A; Tanaka, N; Rossmann, MG

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