Rapid Swelling of a CHO-K1 Aspartate/Glutamate Transport Mutant in Hypo-osmotic Medium

Rapid Swelling of a CHO-K1 Aspartate/Glutamate Transport Mutant in Hypo-osmotic Medium Two Chinese hamster ovary cell (CHO-K1) mutants selected for defective glutamate transport via system X− AG are also highly permeable to small neutral molecules. Light microscopy demonstrated that exposure of one of these mutants, Ed-A1, to hypo-osmotic medium led to extremely rapid swelling, presumably due to increased water flux. When placed in 20% saline, Ed-A1 cells swelled to three times their original volume within 15 sec, a sixfold larger increase than parental CHO-K1. In spite of this rapid volume increase, mutant and wild-type cells remained viable for 20 min in dilute saline. A regulatory volume decrease in Ed-A1, and the continual swelling of CHO-K1, resulted in the two cells achieving equal size after 5 min in 20% saline. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Rapid Swelling of a CHO-K1 Aspartate/Glutamate Transport Mutant in Hypo-osmotic Medium

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Copyright © Inc. by 1997 Springer-Verlag New York
Life Sciences; Biochemistry, general; Human Physiology
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