Ichthyol Res (2018) 65:276–281 https://doi.org/10.1007/s10228-017-0609-0 NEWS AND COMMENTS Rapid detection of alien DNA in the loach genus Misgurnus (family Cobitidae) using real‑time PCR 1 1 2 Takaaki Shimizu · Kimiyoshi Narita · Norio Onikura Received: 5 March 2017 / Revised: 6 October 2017 / Accepted: 24 October 2017 / Published online: 8 November 2017 © The Ichthyological Society of Japan 2017 Cryptic alien introduction of non-native species and genetic mitochondrial clades, which were designated as Clades A, material into native populations can disrupt assessment B and C. However, subsequent reports in the literature have of species diversity and population structure, and imple- shown that both Clades B and C of Koizumi et al. (2009) mentation of conservation programs. Interspecific alien actually belong to Clade B of Morishima et al. (2008), and introduction among morphologically indistinct congeners are therefore, termed Clades B2 and B1 (Shimizu and Tak- often occurs cryptically, and leads to irreversible ecologi- agi 2010a, b). In this study, we followed the A, B1, and cal disturbances (Konishi 2010). Intraspecific immigration B2 nomenclature. The members of Clades B1 and B2, are among local populations also complicates the natural history distributed throughout the Japanese archipelago, Clade A of resident populations (Takehana 2010). In Japan, exotic is restricted
Ichthyological Research – Springer Journals
Published: Nov 8, 2017
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