Mammalian Genome 9, 157-159 (1998). Mammalian Genome Incu1PO 11.9 V ^rsr 0 Gen © Springer-Verlag New York Inc. 1998 Rapid cloning of mouse DNA as yeast artificial chromosomes by transformation-associated recombination (TAR) 3 R.H. Reeves,3 K.M. Tainton, 2 K.H.A. Choo, 2 M.A. Resnick,' M.R. Cancilla, 1 '2 J. Graves,' L.E. Matesic, V.L. Larionov,' Natalya Y. Kouprina' 'Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Box 12233, Research Triangle Park, North Carolina 27709, USA 2The Murdoch Institute for Research into Birth Defects, Royal Children's Hospital, Flemington Road, Parkville 3052, Australia 'Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA Received: 27 August 1997 / Accepted: 10 Oct 1997 Several yeast artificial chromosome (YAC) libraries have been gene is deleted) or from the strain YPH857-01 (the entire LYS2 constructed for physical mapping and characterization of the coding sequence and promoter region are deleted) were co- mouse genome (Larin et al. 1991; Chartier et al. 1992; Haldi et al. transformed with TAR vectors plus various genomic DNAs. As 1996). These complex libraries were constructed by in vitro liga- shown in Table 1, transformation of yeast spheroplasts by a mix- tion of telomere-containing vectors to mouse chromosome
Mammalian Genome – Springer Journals
Published: Mar 28, 2009
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