Short Communication Incorporating Mouse Genome Mammalian Genome 13, 223–226 (2002). © Springer-Verlag New York Inc. 2002 DOI: 10.1007/s00335-001-2128-5 Rapid and efficient detection of transgene homozygosity by FISH of mouse fibroblasts 1 1 1 2 1 Laura Shapiro Kulnane, Emily J.H. Lehman, Brian J. Hock, Karen D. Tsuchiya, Bruce T. Lamb Department of Genetics and Neuroscience, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106-4955, USA and Center for Human Genetics, University Alzheimer Center and Ireland Cancer Center, University Hospitals of Cleveland, Cleveland, Ohio 44106, USA Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA Received: 25 September 2001 / Accepted: 17 December 2001 The generation of homozygous transgenic mice has a number of Southern blot analysis, this requires the isolation of genomic DNA, extremely useful applications in modern biological research. First, restriction enzyme digestion, gel electrophoresis, Southern trans- a homozygous transgene is generally expressed at a higher level fer, hybridization with radioactive DNA probes, and detection and when compared with the corresponding hemizygous state and of- quantification of radioactivity, all of which takes ∼1–2 weeks. ten leads to altered phenotypes (see Lamb et al. 1999; Leavitt et al. Progeny testing of potential homozygotes requires an additional
Mammalian Genome – Springer Journals
Published: Feb 12, 2014
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