Rapid and efficient detection of transgene homozygosity by FISH of mouse fibroblasts

Rapid and efficient detection of transgene homozygosity by FISH of mouse fibroblasts Short Communication Incorporating Mouse Genome Mammalian Genome 13, 223–226 (2002). © Springer-Verlag New York Inc. 2002 DOI: 10.1007/s00335-001-2128-5 Rapid and efficient detection of transgene homozygosity by FISH of mouse fibroblasts 1 1 1 2 1 Laura Shapiro Kulnane, Emily J.H. Lehman, Brian J. Hock, Karen D. Tsuchiya, Bruce T. Lamb Department of Genetics and Neuroscience, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106-4955, USA and Center for Human Genetics, University Alzheimer Center and Ireland Cancer Center, University Hospitals of Cleveland, Cleveland, Ohio 44106, USA Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA Received: 25 September 2001 / Accepted: 17 December 2001 The generation of homozygous transgenic mice has a number of Southern blot analysis, this requires the isolation of genomic DNA, extremely useful applications in modern biological research. First, restriction enzyme digestion, gel electrophoresis, Southern trans- a homozygous transgene is generally expressed at a higher level fer, hybridization with radioactive DNA probes, and detection and when compared with the corresponding hemizygous state and of- quantification of radioactivity, all of which takes ∼1–2 weeks. ten leads to altered phenotypes (see Lamb et al. 1999; Leavitt et al. Progeny testing of potential homozygotes requires an additional http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

Rapid and efficient detection of transgene homozygosity by FISH of mouse fibroblasts

Loading next page...
 
/lp/springer_journal/rapid-and-efficient-detection-of-transgene-homozygosity-by-fish-of-YefrjBuK8C
Publisher
Springer-Verlag
Copyright
Copyright © 2002 by Springer-Verlag New York Inc.
Subject
Life Sciences; Cell Biology; Anatomy; Zoology
ISSN
0938-8990
eISSN
1432-1777
D.O.I.
10.1007/s00335-001-2128-5
Publisher site
See Article on Publisher Site

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial