Rabbit hemorrhagic disease virus poly(A) tail is not essential for the infectivity of the virus and can be restored in vivo

Rabbit hemorrhagic disease virus poly(A) tail is not essential for the infectivity of the virus... The precise role of the poly(A) tail at the 3′-end of the calicivirus RNA genome is unknown. To study the relationship between the presence of the poly(A) tail and the infectivity and replication of rabbit hemorrhagic disease virus (RHDV), mutants of an infectious cDNA clone of RHDV were constructed, and RK13 cells were transfected with transcripts from these mutants. Transcripts with and without a poly(A) had a fairly similar ability to infect and replicate, suggesting that a long 3′-terminal poly(A) is not essential for infectivity and replication. RT-PCR with specific primers, using viral RNA recovered from RK13 cells transfected with poly(A)-deficient RNA transcripts, showed that the poly(A) tail was restored in vivo. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Rabbit hemorrhagic disease virus poly(A) tail is not essential for the infectivity of the virus and can be restored in vivo

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Publisher
Springer Vienna
Copyright
Copyright © 2008 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-008-0079-9
Publisher site
See Article on Publisher Site

Abstract

The precise role of the poly(A) tail at the 3′-end of the calicivirus RNA genome is unknown. To study the relationship between the presence of the poly(A) tail and the infectivity and replication of rabbit hemorrhagic disease virus (RHDV), mutants of an infectious cDNA clone of RHDV were constructed, and RK13 cells were transfected with transcripts from these mutants. Transcripts with and without a poly(A) had a fairly similar ability to infect and replicate, suggesting that a long 3′-terminal poly(A) is not essential for infectivity and replication. RT-PCR with specific primers, using viral RNA recovered from RK13 cells transfected with poly(A)-deficient RNA transcripts, showed that the poly(A) tail was restored in vivo.

Journal

Archives of VirologySpringer Journals

Published: May 1, 2008

References

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