Quasi-adaptive response to alkylating agents and Ada-protein functions in Escherichia coli

Quasi-adaptive response to alkylating agents and Ada-protein functions in Escherichia coli In the exponentially growing E. coli cells we have described in 2005 a new fundamental genetic phenomenon, namely quasi-adaptive response to alkylating compounds, “quasi-Ada”. Phenotypic expression of “quasi-Ada” is similar to the true Ada response, however in contrast it develops in the course of pretreatment of the cells by sublethal dose of non-alkylating agent, an NO-containing dinitrosyl iron complex with glutathione (DNICglu). To reveal the mechanisms of quasi-adaptation and its association with the function of the regulatory protein Ada here we used a unique property of dual gene expression regulation of aidB1 gene, a part of Ada-regulon, namely its relative independence from Ada protein in anaerobic conditions. Based on the results of aidB1 gene expression analysis an EPR spectra of E.coli MV2176 cells (aidB1::lacZ) in aerobic and anaerobic conditions after the corresponding treatments we concluded that the function and the spatial structure of meAda and [(Cys−)2Fe+(NO+)2]Ada are identical and thus the nitrosylated protein represents an Ada regulon genes expression regulator during quasi-adaptation development. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Quasi-adaptive response to alkylating agents and Ada-protein functions in Escherichia coli

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Copyright © 2008 by Pleiades Publishing, Inc.
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
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