Quantitative regulation of the Flaveria Me1 gene is controlled by the 3′-untranslated region and sequences near the amino terminus

Quantitative regulation of the Flaveria Me1 gene is controlled by the 3′-untranslated region... The Me1 gene of Flaveria bidentis codes for the C4 isoform of NADP malic enzyme, which accumulates to a high-level only in bundle sheath cells. Previous experiments demonstrated that sequences at the 5′ end of the gene control cell specificity whereas sequences at the 3′ end are necessary for high-level expression. To localize quantitative regulator sequences, we have analysed a series of Me1 3′ deletion constructs fused to the gusA reporter gene. We show that sequences within the 3′-untranslated region (3′-UTR) control quantitative levels of expression. Analysis of 5′ promoter fusions demonstrated that high-level expression also requires sequences within the N-terminal coding region of the gene, suggesting possible interactions between the 3′-UTR and 5′ coding regions. Cell-specific regulatory sequences are located in a different part of the 5′ end of the gene, between 1023 bp upstream of the transcription start and the start of translation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Quantitative regulation of the Flaveria Me1 gene is controlled by the 3′-untranslated region and sequences near the amino terminus

Loading next page...
 
/lp/springer_journal/quantitative-regulation-of-the-flaveria-me1-gene-is-controlled-by-the-FaxsW060Ds
Publisher
Kluwer Academic Publishers
Copyright
Copyright © 2001 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1010684509008
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial