Quantitative characterization of nitrogen regulation of OsAMT1;1, OsAMT1;2, and OsAMT2;2 expression in rice seedlings

Quantitative characterization of nitrogen regulation of OsAMT1;1, OsAMT1;2, and OsAMT2;2... The effects of nitrogen forms and supply levels on rice ammonium transporter expression have previously been studied by Northern blot or semiquantitative PCR in a model rice Oryza sativa L., cv. Nipponbare. However, most ammonium transporters (AMT) have low abundance in rice, and it is difficult to accurately analyze their transcript levels. In this study, an analysis of the transcript levels of the OsAMT1;1, OsAMT1;2, and OsAMT2;2 in rice seedlings, cv. Guidan 4 has been performed under various nitrogen conditions, using the technique of real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). The OsAMT1;1 and OsAMT2;2 mRNA levels in nitrogen prestarved plants were induced by supply of NH 4 + , NH4NO3, and NO 3 − , while OsAMT1;2 mRNA in roots was repressed by the same treatment. Expression of OsAMT1;2 in nitrogen-starved rice shoots was induced steeply when rice plants were exposed to 0.5 mM NH4NO3 or 2 mM NH 4 + -N whereas both OsAMT1;1 and OsAMT2;2 exhibited only modest changes under same conditions. OsAMT1;1 and OsAMT1;2 were expressed preferentially in roots while OsAMT2;2 was expressed evenly in roots and shoots, suggesting that OsAMT2;2 may play a different physiological role in ammonium uptake. The upregulation effect of NH4NO3 on OsAMT1;1 mRNA in roots is between that of NO 3 − and NH 4 + , and its induction effect on OsAMT2;2 mRNA level is higher than that of NO 3 − or NH 4 + in both roots and shoots. These three OsAMTs genes in the Guidan 4 rice cultivar show dissimilar transcriptional regulation by various levels of different nitrogen species, and the nitrogen-regulated expression patterns are different from those of the model rice cv. Nipponbare. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Quantitative characterization of nitrogen regulation of OsAMT1;1, OsAMT1;2, and OsAMT2;2 expression in rice seedlings

Loading next page...
 
/lp/springer_journal/quantitative-characterization-of-nitrogen-regulation-of-osamt1-1-ya03JAR6ij
Publisher
Nauka/Interperiodica
Copyright
Copyright © 2006 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S102144370606015X
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial