Quantification of Trypsin Activity by a New Biosensing System Based on the Enzymatic Degradation and the Destructive Nature of Trypsin

Quantification of Trypsin Activity by a New Biosensing System Based on the Enzymatic Degradation... Trypsin is an enzyme that is included in the hydrolysis class of enzymes. Trypsin catalyzes the cleavage of proteins from a serine residue and is found in the digestive system of vertebrates. The cystic fibrosis, which is an autosomal recessive disorder, is associated with the trypsin deficiency. The meconium ileus is caused by this deficiency. Beside the clinical significance of trypsin, it is also used in many industrial applications, such as tissue culture labs, milk industry, proteomics, and different kinds of food production processes. In this study, a glucose oxidase based electrochemical biosensing system was constructed for sensitive and accurate determination of trypsin activity in different samples. A novel working principle was operated in the reported biosensor, which is based on the trypsin degradation ratio in the bioactive layer of the biosensor. Detailed optimization and characterization experiments were carried out to obtain the best electrochemical signals. The system introduced a linear determination range for trypsin activity between 6.65 and 33.25 U/ml. The biosensor was also used in the analysis of the trypsin activities of real samples. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Peptide Research and Therapeutics Springer Journals

Quantification of Trypsin Activity by a New Biosensing System Based on the Enzymatic Degradation and the Destructive Nature of Trypsin

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Publisher
Springer Netherlands
Copyright
Copyright © 2016 by Springer Science+Business Media New York
Subject
Life Sciences; Biochemistry, general; Animal Anatomy / Morphology / Histology; Polymer Sciences; Pharmaceutical Sciences/Technology; Pharmacology/Toxicology; Molecular Medicine
ISSN
1573-3149
eISSN
1573-3904
D.O.I.
10.1007/s10989-016-9563-3
Publisher site
See Article on Publisher Site

Abstract

Trypsin is an enzyme that is included in the hydrolysis class of enzymes. Trypsin catalyzes the cleavage of proteins from a serine residue and is found in the digestive system of vertebrates. The cystic fibrosis, which is an autosomal recessive disorder, is associated with the trypsin deficiency. The meconium ileus is caused by this deficiency. Beside the clinical significance of trypsin, it is also used in many industrial applications, such as tissue culture labs, milk industry, proteomics, and different kinds of food production processes. In this study, a glucose oxidase based electrochemical biosensing system was constructed for sensitive and accurate determination of trypsin activity in different samples. A novel working principle was operated in the reported biosensor, which is based on the trypsin degradation ratio in the bioactive layer of the biosensor. Detailed optimization and characterization experiments were carried out to obtain the best electrochemical signals. The system introduced a linear determination range for trypsin activity between 6.65 and 33.25 U/ml. The biosensor was also used in the analysis of the trypsin activities of real samples.

Journal

International Journal of Peptide Research and TherapeuticsSpringer Journals

Published: Nov 1, 2016

References

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