Qualitative differences in brain-infiltrating T cells are associated with a fatal outcome in mice infected with Japanese encephalitis virus

Qualitative differences in brain-infiltrating T cells are associated with a fatal outcome in mice... Japanese encephalitis (JE) is the most important form of viral encephalitis in Asia. The critical factors determining mortality and severity of JE virus (JEV) infection remain unclear. We identified brain-infiltrating T cells associated with a fatal outcome of JEV infection in mice. Dying mice were defined as those that lost more than 25 % of their body weight by day 13 and died by day 21, while surviving mice were defined as those that lost less than 10 % by day 13, based on the result of the survival time course study. Two groups of five mice that demonstrated brain virus titers of >1 × 10 6 pfu/g were randomly selected from the dying and surviving groups and used in the analyses. Cytokine patterns in brains were first examined, revealing a higher ratio of Th1-related cytokine genes in dying mice. The expression levels of CD3, CD8, CD25, and CD69 increased in JEV-infected mice relative to mock-infected mice. However, expression levels of these cell-surface markers did not differ between the two groups. T-cell receptor (TCR) usage and complementary determining region 3 (CDR3) sequences were analyzed in the brain-infiltrating T cells. T cells expressing VA8-1, VA10-1, and VB2-1 increased in both groups. However, the dominant T-cell clones as defined by CDR3 amino acid sequence differed between the two groups. The results indicate that the outcome of JEV infection, death or survival, was determined by qualitative differences in infiltrating T-cell clones with unique CDR3 amino acid sequences. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Qualitative differences in brain-infiltrating T cells are associated with a fatal outcome in mice infected with Japanese encephalitis virus

Loading next page...
 
/lp/springer_journal/qualitative-differences-in-brain-infiltrating-t-cells-are-associated-yMC0WKKXiY
Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2154-8
Publisher site
See Article on Publisher Site

References

  • Chemokine receptor CCR5 promotes leukocyte trafficking to the brain and survival in West Nile virus infection
    Glass, WG; Lim, JK; Cholera, R; Pletnev, AG; Gao, JL
  • Protective capacity and epitope specificity of CD8(+) T cells responding to lethal West Nile virus infection
    Brien, JD; Uhrlaub, JL; Nikolich-Zugich, J
  • Antigen-specific cytotoxic T lymphocytes protect against lethal West Nile virus encephalitis
    Purtha, WE; Myers, N; Mitaksov, V; Sitati, E; Connolly, J
  • An instructive component in T helper cell type 2 (Th2) development mediated by GATA-3
    Farrar, JD; Ouyang, W; Lohning, M; Assenmacher, M; Radbruch, A
  • Mouse CD1-specific NK1 T cells: development, specificity, and function
    Bendelac, A; Rivera, MN; Park, SH; Roark, JH
  • Definition of an epitope on Japanese encephalitis virus (JEV) envelope protein recognized by JEV-specific murine CD8+ cytotoxic T lymphocytes
    Takada, K; Masaki, H; Konishi, E; Takahashi, M; Kurane, I
  • CD4+CD25+ T cells regulate virus-specific primary and memory CD8+ T cell responses
    Suvas, S; Kumaraguru, U; Pack, CD; Lee, S; Rouse, BT
  • An immunomodulatory role for CD4(+)CD25(+) regulatory T lymphocytes in hepatitis C virus infection
    Cabrera, R; Tu, Z; Xu, Y; Firpi, RJ; Rosen, HR

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial