Pyramiding of transgenic Pm3 alleles in wheat results in improved powdery mildew resistance in the field

Pyramiding of transgenic Pm3 alleles in wheat results in improved powdery mildew resistance in... Key message The combined effects of enhanced total transgene expression level and allele-specificity combination in transgenic allele-pyramided Pm3 wheat lines result in improved powdery mildew field resistance without negative pleiotropic effects. Abstract Allelic Pm3 resistance genes of wheat confer race-specific resistance to powdery mildew ( Blumeria graminis f. sp. tritici, Bgt) and encode nucleotide-binding domain, leucine-rich repeat (NLR) receptors. Transgenic wheat lines overexpress- ing alleles Pm3a, b, c, d, f, and g have previously been generated by transformation of cultivar Bobwhite and tested in field trials, revealing varying degrees of powdery mildew resistance conferred by the transgenes. Here, we tested four transgenic lines each carrying two pyramided Pm3 alleles, which were generated by crossbreeding of lines transformed with single Pm3 alleles. All four allele-pyramided lines showed strongly improved powdery mildew resistance in the field compared to their parental lines. The improved resistance results from the two effects of enhanced total transgene expression levels and allele-specificity combinations. In contrast to leaf segment tests on greenhouse-grown seedlings, no allelic suppression was observed in the field. Plant development and yield scores of the pyramided lines were similar to the mean scores of the corre - sponding parental lines, and thus, the allele http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png TAG Theoretical and Applied Genetics Springer Journals

Pyramiding of transgenic Pm3 alleles in wheat results in improved powdery mildew resistance in the field

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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2018 by The Author(s)
Subject
Life Sciences; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Agriculture; Plant Biochemistry; Biochemistry, general; Biotechnology
ISSN
0040-5752
eISSN
1432-2242
D.O.I.
10.1007/s00122-017-3043-9
Publisher site
See Article on Publisher Site

Abstract

Key message The combined effects of enhanced total transgene expression level and allele-specificity combination in transgenic allele-pyramided Pm3 wheat lines result in improved powdery mildew field resistance without negative pleiotropic effects. Abstract Allelic Pm3 resistance genes of wheat confer race-specific resistance to powdery mildew ( Blumeria graminis f. sp. tritici, Bgt) and encode nucleotide-binding domain, leucine-rich repeat (NLR) receptors. Transgenic wheat lines overexpress- ing alleles Pm3a, b, c, d, f, and g have previously been generated by transformation of cultivar Bobwhite and tested in field trials, revealing varying degrees of powdery mildew resistance conferred by the transgenes. Here, we tested four transgenic lines each carrying two pyramided Pm3 alleles, which were generated by crossbreeding of lines transformed with single Pm3 alleles. All four allele-pyramided lines showed strongly improved powdery mildew resistance in the field compared to their parental lines. The improved resistance results from the two effects of enhanced total transgene expression levels and allele-specificity combinations. In contrast to leaf segment tests on greenhouse-grown seedlings, no allelic suppression was observed in the field. Plant development and yield scores of the pyramided lines were similar to the mean scores of the corre - sponding parental lines, and thus, the allele

Journal

TAG Theoretical and Applied GeneticsSpringer Journals

Published: Jan 4, 2018

References

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