Purification and Characterization of Extracellular Lipase from Serratia marcescens VITSD2

Purification and Characterization of Extracellular Lipase from Serratia marcescens VITSD2 Lipases represent a group of extracellular enzymes and hold a prominent place among the enzymes. In the present research work Serratia marcescens isolated from soil was selected for lipase production. Effects of various parameters such as substrate, pH, temperature, incubation time and inoculum size were analysed for the enhanced lipase production. The optimum substrate was found to be olive oil with the concentration of 0.7 mL in 100 mL of production medium. Serratia marcescens produced maximum lipase after 48 h of incubation at 30 °C with the optimum pH as 7.0. The inoculum concentration of 4 % was more favourable for the production of lipase. The extracted lipase was purified by two steps: ultra-filtration and Carboxymethyl(CM)-Cellulose chromatography. The specific activity of the purified enzyme was determined to be 65.0 U/mg with 2.55 purification fold and yield of 67.5 %. Polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed the presence of a single band with a molecular weight of around 35 kDa. Based on Fourier transformation-infrared spectroscopy analysis the functional groups of lipase were determined and high performance liquid chromatography analysis with the retention time of 1.995 min reveals the presence of lipase in the purified extract. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

Purification and Characterization of Extracellular Lipase from Serratia marcescens VITSD2

Loading next page...
 
/lp/springer_journal/purification-and-characterization-of-extracellular-lipase-from-VFsYKv8tfL
Publisher
Springer India
Copyright
Copyright © 2016 by The National Academy of Sciences, India
Subject
Life Sciences; Life Sciences, general; Behavioral Sciences; Plant Biochemistry; Nucleic Acid Chemistry
ISSN
0369-8211
eISSN
2250-1746
D.O.I.
10.1007/s40011-016-0763-6
Publisher site
See Article on Publisher Site

Abstract

Lipases represent a group of extracellular enzymes and hold a prominent place among the enzymes. In the present research work Serratia marcescens isolated from soil was selected for lipase production. Effects of various parameters such as substrate, pH, temperature, incubation time and inoculum size were analysed for the enhanced lipase production. The optimum substrate was found to be olive oil with the concentration of 0.7 mL in 100 mL of production medium. Serratia marcescens produced maximum lipase after 48 h of incubation at 30 °C with the optimum pH as 7.0. The inoculum concentration of 4 % was more favourable for the production of lipase. The extracted lipase was purified by two steps: ultra-filtration and Carboxymethyl(CM)-Cellulose chromatography. The specific activity of the purified enzyme was determined to be 65.0 U/mg with 2.55 purification fold and yield of 67.5 %. Polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed the presence of a single band with a molecular weight of around 35 kDa. Based on Fourier transformation-infrared spectroscopy analysis the functional groups of lipase were determined and high performance liquid chromatography analysis with the retention time of 1.995 min reveals the presence of lipase in the purified extract.

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological SciencesSpringer Journals

Published: Jul 22, 2016

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from Google Scholar, PubMed
Create lists to organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off