Pst DC3000 induces pathogenesis-uncorrelated cytosolic Ca2+ rise in Arabidopsis leaves

Pst DC3000 induces pathogenesis-uncorrelated cytosolic Ca2+ rise in Arabidopsis leaves In nature, the phytopathogen usually initiates its infection on the leaf surface before moving into the internal space through natural openings. Little is known about immediate response of the leaf to the surface-colonizing phytopathogen and its correlation with individual microbe-associated molecular patterns (MAMPs). In this study, we monitored the dynamic changes in the cytosolic Ca2+ concentration ([Ca2+]cyt) in the Arabidopsis leaf expressing luminescence protein aequorin as the response to the surface-inoculating Pseudomonas syringae DC3000 (Pst DC3000) with a touching-free system. The significant [Ca2+]cyt transient rise was evoked in the leaf right after inoculation, and its magnitude was correlated with the pathogen concentration. Pharmacological studies revealed that the rising [Ca2+]cyt occurs primarily from the cAMP-mediated Ca2+ mobility pathway, but not Gd3+-sensitive Ca2+ influx channel in the plasma membrane, which was distinct from those induced by individual MAMPs (lipopolysaccharide, flagellin, and elongation factor Tu). Pretreating the leaf with Pst DC3000 or MAMPs significantly attenuated its responses to subsequent treatments of any of them, which indicates that the leaf has the convergent mechanism of sensitivity to the pathogen and MAMPs. Furthermore, Pst DC3000 mutants defective in flagellum, type III secretion apparatus, and phytotoxin coronine production significantly lost their multiplication ability in the leaf apoplast, but evoked [Ca2+]cyt responses comparable with that of the wild type. Taken together, these data indicates that the [Ca2+]cyt in the leaf has the sensitive response to the surface-inoculating phytopathogen, which was distinct from those of individual MAMPs and had no correlation with the pathogen pathogenesis capacity. Russian Journal of Plant Physiology Springer Journals

Pst DC3000 induces pathogenesis-uncorrelated cytosolic Ca2+ rise in Arabidopsis leaves

Loading next page...
Pleiades Publishing
Copyright © 2014 by Pleiades Publishing, Ltd.
Life Sciences; Plant Physiology; Plant Sciences
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial