Pst DC3000 induces pathogenesis-uncorrelated cytosolic Ca2+ rise in Arabidopsis leaves

Pst DC3000 induces pathogenesis-uncorrelated cytosolic Ca2+ rise in Arabidopsis leaves In nature, the phytopathogen usually initiates its infection on the leaf surface before moving into the internal space through natural openings. Little is known about immediate response of the leaf to the surface-colonizing phytopathogen and its correlation with individual microbe-associated molecular patterns (MAMPs). In this study, we monitored the dynamic changes in the cytosolic Ca2+ concentration ([Ca2+]cyt) in the Arabidopsis leaf expressing luminescence protein aequorin as the response to the surface-inoculating Pseudomonas syringae DC3000 (Pst DC3000) with a touching-free system. The significant [Ca2+]cyt transient rise was evoked in the leaf right after inoculation, and its magnitude was correlated with the pathogen concentration. Pharmacological studies revealed that the rising [Ca2+]cyt occurs primarily from the cAMP-mediated Ca2+ mobility pathway, but not Gd3+-sensitive Ca2+ influx channel in the plasma membrane, which was distinct from those induced by individual MAMPs (lipopolysaccharide, flagellin, and elongation factor Tu). Pretreating the leaf with Pst DC3000 or MAMPs significantly attenuated its responses to subsequent treatments of any of them, which indicates that the leaf has the convergent mechanism of sensitivity to the pathogen and MAMPs. Furthermore, Pst DC3000 mutants defective in flagellum, type III secretion apparatus, and phytotoxin coronine production significantly lost their multiplication ability in the leaf apoplast, but evoked [Ca2+]cyt responses comparable with that of the wild type. Taken together, these data indicates that the [Ca2+]cyt in the leaf has the sensitive response to the surface-inoculating phytopathogen, which was distinct from those of individual MAMPs and had no correlation with the pathogen pathogenesis capacity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Pst DC3000 induces pathogenesis-uncorrelated cytosolic Ca2+ rise in Arabidopsis leaves

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Publisher
Springer Journals
Copyright
Copyright © 2014 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443714030133
Publisher site
See Article on Publisher Site

Abstract

In nature, the phytopathogen usually initiates its infection on the leaf surface before moving into the internal space through natural openings. Little is known about immediate response of the leaf to the surface-colonizing phytopathogen and its correlation with individual microbe-associated molecular patterns (MAMPs). In this study, we monitored the dynamic changes in the cytosolic Ca2+ concentration ([Ca2+]cyt) in the Arabidopsis leaf expressing luminescence protein aequorin as the response to the surface-inoculating Pseudomonas syringae DC3000 (Pst DC3000) with a touching-free system. The significant [Ca2+]cyt transient rise was evoked in the leaf right after inoculation, and its magnitude was correlated with the pathogen concentration. Pharmacological studies revealed that the rising [Ca2+]cyt occurs primarily from the cAMP-mediated Ca2+ mobility pathway, but not Gd3+-sensitive Ca2+ influx channel in the plasma membrane, which was distinct from those induced by individual MAMPs (lipopolysaccharide, flagellin, and elongation factor Tu). Pretreating the leaf with Pst DC3000 or MAMPs significantly attenuated its responses to subsequent treatments of any of them, which indicates that the leaf has the convergent mechanism of sensitivity to the pathogen and MAMPs. Furthermore, Pst DC3000 mutants defective in flagellum, type III secretion apparatus, and phytotoxin coronine production significantly lost their multiplication ability in the leaf apoplast, but evoked [Ca2+]cyt responses comparable with that of the wild type. Taken together, these data indicates that the [Ca2+]cyt in the leaf has the sensitive response to the surface-inoculating phytopathogen, which was distinct from those of individual MAMPs and had no correlation with the pathogen pathogenesis capacity.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Apr 27, 2014

References

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