PsbS expression analysis in two ecotypes of Sedum alfredii and the role of SaPsbS in Cd tolerance

PsbS expression analysis in two ecotypes of Sedum alfredii and the role of SaPsbS in Cd tolerance The effects of Cd on the chlorophyll fluorescence parameters of hyperaccumulating ecotype (HE) and non-hyperaccumulating ecotype (NHE) of Sedum alfredii Hance were studied. The photosystem II (PSII) photochemical efficiency of HE plants was not affected, and the non-photochemical quenching (NPQ) value was significantly increased under Cd treatment. In the NHE plants, Cd treatment caused significant loss in PSII photochemical efficiency and NPQ value. Gene expression analysis showed that both ecotypes of S. alfredii accumulated more than twofold higher PsbS (PSII subunit S) transcript levels than control plants after exposure to Cd. Overexpression of PsbS gene isolated from HE plants (SaPsbS) enhanced tobacco growth. The transgenic tobacco possessed greater NPQ capacity in the presence of 100 μM Cd and accumulated the significantly higher Cd content in shoots than the wild-type plants. These data indicate that the SaPsbS may function in NPQ capacity and protect the PSII reaction center in HE plants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

PsbS expression analysis in two ecotypes of Sedum alfredii and the role of SaPsbS in Cd tolerance

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Publisher
Springer Journals
Copyright
Copyright © 2014 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443714040219
Publisher site
See Article on Publisher Site

Abstract

The effects of Cd on the chlorophyll fluorescence parameters of hyperaccumulating ecotype (HE) and non-hyperaccumulating ecotype (NHE) of Sedum alfredii Hance were studied. The photosystem II (PSII) photochemical efficiency of HE plants was not affected, and the non-photochemical quenching (NPQ) value was significantly increased under Cd treatment. In the NHE plants, Cd treatment caused significant loss in PSII photochemical efficiency and NPQ value. Gene expression analysis showed that both ecotypes of S. alfredii accumulated more than twofold higher PsbS (PSII subunit S) transcript levels than control plants after exposure to Cd. Overexpression of PsbS gene isolated from HE plants (SaPsbS) enhanced tobacco growth. The transgenic tobacco possessed greater NPQ capacity in the presence of 100 μM Cd and accumulated the significantly higher Cd content in shoots than the wild-type plants. These data indicate that the SaPsbS may function in NPQ capacity and protect the PSII reaction center in HE plants.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Jun 24, 2014

References

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