The pathogenesis of natural scrapie in Sarda breed sheep was investigated in 1050 asymptomatic and 49 sick sheep from scrapie-affected flocks. Central and peripheral nervous system, along with lymphoreticular system (LRS) tissues, were subjected to immunohistochemistry (IHC) and Western-blotting (WB) for detection of pathological isoform of the prion protein (PrP Sc ). A total of 69 of the 1050 clinically healthy sheep were found to be infected with scrapie, with PrP Sc being detected in both the central nervous system (CNS) and enteric nervous system (ENS) plexuses of 60 of the sheep, while IHC and WB yielded evidence of (PrP Sc ) deposition only in lymphoid tissues of the remaining 9 clinically healthy sheep. PrP Sc was also detected in the CNS, as well as in ENS plexuses from all of the 49 clinically affected sheep. Nevertheless, 18 of the 69 clinically healthy animals (26%, 17 ARQ/ARQ and 1 ARQ/AHQ sheep), along with 3 ARQ/ARQ sheep (6%) of the clinically affected group, showed no IHC or WB evidence of PrP Sc in lymphoid tissues, but PrP Sc could be still detected in their CNS and ENS plexuses. The study demonstrates dual CNS and ENS PrP Sc deposition in Sarda sheep with scrapie, in spite of an apparent lack of lymphoid tissue involvement in a number of cases.
Archives of Virology – Springer Journals
Published: Oct 1, 2006
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