Proteomic analysis of temperature dependency of buckwheat seed dormancy and quality degradation

Proteomic analysis of temperature dependency of buckwheat seed dormancy and quality degradation Buckwheat is a functional staple food, which is rich in rutin and other flavonoids with strong antioxidant potential. However, the quality of buckwheat grain is easy to degrade during storage and low temperature is better to keep the flavor and texture. We tested the hypothesis that the quality degradation is related to seed dormancy which is lost at a rate in temperature-dependent manner in most seeds. To understand physiological processes in seeds under temperature stress, which affects quality of stored buckwheat grain, a gel-free/label-free proteomic analysis was conducted. Compared to the seeds before storage, a total of 30, 76, 52, 14, and 61 proteins were affected at 5 °C for 5 months, 5 °C for 10 months, 15 °C for 5 months, 15 °C for 10 months, and 25 °C for 5 months, respectively. Functional classification revealed that storage condition at 5 °C for 5 months resulted in different protein profile from other conditions and suitable for keeping best condition. Storage at higher temperature for longer time influenced proteins related to preparation for emergence by carbohydrate metabolism, endogenous gene expression, and protein homeostasis. Proteins such as granule-bound starch synthase and 13S seed storage protein consistently decreased in abundance during storage. These results suggest that stored buckwheat seeds experience seed dormancy release, but the processes are not clear in relation to temperature rise. Dormancy break is recognized but emergence is inhibited by abscisic acid, which was induced by temperature stress, showing the complexity of buckwheat germination control. The quality degradation of stored seeds might be due to changed starch composition and protein network. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Theoretical and Experimental Plant Physiology Springer Journals

Proteomic analysis of temperature dependency of buckwheat seed dormancy and quality degradation

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Brazilian Society of Plant Physiology
Subject
Life Sciences; Life Sciences, general
eISSN
2197-0025
DOI
10.1007/s40626-018-0104-7
Publisher site
See Article on Publisher Site

Abstract

Buckwheat is a functional staple food, which is rich in rutin and other flavonoids with strong antioxidant potential. However, the quality of buckwheat grain is easy to degrade during storage and low temperature is better to keep the flavor and texture. We tested the hypothesis that the quality degradation is related to seed dormancy which is lost at a rate in temperature-dependent manner in most seeds. To understand physiological processes in seeds under temperature stress, which affects quality of stored buckwheat grain, a gel-free/label-free proteomic analysis was conducted. Compared to the seeds before storage, a total of 30, 76, 52, 14, and 61 proteins were affected at 5 °C for 5 months, 5 °C for 10 months, 15 °C for 5 months, 15 °C for 10 months, and 25 °C for 5 months, respectively. Functional classification revealed that storage condition at 5 °C for 5 months resulted in different protein profile from other conditions and suitable for keeping best condition. Storage at higher temperature for longer time influenced proteins related to preparation for emergence by carbohydrate metabolism, endogenous gene expression, and protein homeostasis. Proteins such as granule-bound starch synthase and 13S seed storage protein consistently decreased in abundance during storage. These results suggest that stored buckwheat seeds experience seed dormancy release, but the processes are not clear in relation to temperature rise. Dormancy break is recognized but emergence is inhibited by abscisic acid, which was induced by temperature stress, showing the complexity of buckwheat germination control. The quality degradation of stored seeds might be due to changed starch composition and protein network.

Journal

Theoretical and Experimental Plant Physiology – Springer Journals

Published: May 24, 2018

References

  • Seeds: ecology, biogeography, and evolution of dormancy and germination
    Baskin, CC; Baskin, JM
  • A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
    Bradford, MM
  • Effect of processing on the flavonoid content in buckwheat (Fagopyrum esculentum Moench) grain
    Dietrych-Szostak, D; Oleszek, W
  • The role of carbohydrates in seed germination and seedling establishment of Himatanthus sucuuba, an Amazonian tree with populations adapted to flooded and non-flooded conditions
    Ferreira Cda, S; Piedade, MT; Tiné, MA; Rossatto, DR; Parolin, P; Buckeridge, MS
  • ABA inhibits germination but not dormancy release in mature imbibed seeds of Lolium rigidum Gaud
    Goggin, DE; Steadman, KJ; Emery, RJ; Farrow, SC; Benech-Arnold, RL; Powles, SB
  • Abscisic acid: a versatile phytohormone in plant signaling and beyond
    Gomez-Cadenas, A; Vives, V; Zandalinas, SI; Manzi, M; Sanchez-Perez, AM; Perez-Clemente, RM; Arbona, V
  • The impact of protein cross-linking induced by alkali on the quality of buckwheat noodles
    Guo, XN; Wei, XM; Zhu, KX
  • Cultivar difference in grain yield and preharvest sprouting in buckwheat (Fagopyrum esculentum Moench)
    Hara, T; Matsui, K; Ikoma, H; Tetsuka, T
  • Rheological and pasting properties of buckwheat (Fagopyrum esculentum Moench) flours with and without jet-cooking
    Inglett, GE; Xu, J; Stevenson, DG; Chen, D
  • Exponentially modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein
    Ishihama, Y; Oda, Y; Tabata, T; Sato, T; Nagasu, T; Rappsilber, J; Mann, M
  • Genetic diversity among Japanese indigenous common buckwheat (Fagopyrum esculentum) cultivars as determined from amplified fragment length polymorphism and simple sequence repeat markers and quantitative agronomic traits
    Iwata, H; Imon, K; Tsumura, Y; Ohsawa, R
  • The efficacy of snow room (yukimuro) storage for the preservation of buckwheat grain
    Kamiyama, S; Itoh, M; Oshimi, M; Takiguchi, M; Kushihara, S; Ishiguro, M; Kobayashi, K; Shimojo, S; Watanabe, S; Sone, H
  • Effects of storage temperature on flavor of stone-milled buckwheat flour
    Kawakami, I; Murayama, N; Kawasaki, S; Igasaki, T; Hayashida, Y
  • Influence of storage period on characteristics of stone-milled buckwheat flour
    Kawakami, I; Murayama, N; Kawasaki, S; Igasaki, T; Hayashida, Y
  • Tandem repeat inserts in 13S globulin subunits, the major allergenic storage protein of common buckwheat (Fagopyrum esculentum Moench) seeds
    Khan, N; Takahashi, Y; Katsube-Tanaka, T
  • Label-free quantitative proteomic analysis of abscisic acid effect in early-stage soybean under flooding
    Komatsu, S; Han, C; Nanjo, Y; Altaf-Un-Nahar, M; Wang, K; He, D; Yang, P
  • Mercator: a fast and simple web server for genome scale functional annotation of plant sequence data
    Lohse, M; Nagel, A; Herter, T; May, P; Schroda, M; Zrenner, R; Tohge, T; Fernie, AR; Stitt, M; Usadel, B
  • Immunodetection and characterization of allergenic proteins in common buckwheat (Fagopyrum esculentum)
    Nair, A; Adachi, T
  • Parts per million mass accuracy on an Orbitrap mass spectrometer via lock mass injection into a C-trap
    Olsen, JV; Godoy, LM; Li, G; Macek, B; Mortensen, P; Pesch, R; Makarov, A; Lange, O; Horning, S; Mann, M
  • Diversification of 13S globulins, allergenic seed storage proteins, of common buckwheat
    Sano, M; Nakagawa, M; Oishi, A; Yasui, Y; Katsube-Tanaka, T
  • Isolation and characterization of six abscisic acid-inducible genes from carrot somatic embryos
    Shiota, H; Yang, G; Shen, S; Eun, CH; Watabe, K; Tanaka, I; Kamada, H
  • Two faces of one seed: hormonal regulation of dormancy and germination
    Shu, K; Liu, XD; Xie, Q; He, ZH
  • Rice and buckwheat flour characterisation and its relation to cookie quality
    Torbica, A; Hadnađev, M; Hadnađev, TD
  • Dormancy in higher plants
    Vegis, A
  • A simulation model for seasonal changes in dormancy and germination of weed seeds
    Vleeshouwers, LM; Bouwmeester, HJ
  • Effect of dynamic exclusion duration on spectral count based quantitative proteomics
    Zhang, Y; Wen, Z; Washburn, MP; Florens, L

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