Properties of the Functioning of the Promoter of the Microcin C51 Operon under Different Conditions of Escherichia coliCell Growth

Properties of the Functioning of the Promoter of the Microcin C51 Operon under Different... The level of transcription from the promoter of the microcin C51 operon (P mcc ) depends on the growth phase of Escherichia colicells: transcription proceeds with low efficiency at the exponential phase of growth and with higher efficiency when growth of cells is delayed during entry into the stationary phase. The functioning of P mcc was studied in cells grown in different media by a single-copy construct, which contained the cloned promoter region of the microcin C51 operon and the promoterless lacoperon. A decrease in the rate of cell growth caused by changes in the sole carbon source in minimal medium correlated with an increase in the level of transcription from the P mcc promoter at the exponential phase of growth; the expression of P mcc –lacduring cell entry into the stationary phase was higher under less favorable medium conditions. The use of composite rich media impaired this feature. The addition of l-leucine (100 μg/ml) to the medium decreased the expression of P mcc –lacin wild-type cells and those carrying the Δlrpmutation. A further increase in leucine concentration and the presence of other amino acids in the medium enhanced transcription that started from P mcc during cell entry into the stationary growth phase. The capacity of the P mcc promoter and of the wild-type lacZgene promoter upon IPTG induction was virtually the same. A mutation in the ompRgene did not markedly influence transcription started from P mcc . http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Properties of the Functioning of the Promoter of the Microcin C51 Operon under Different Conditions of Escherichia coliCell Growth

Loading next page...
 
/lp/springer_journal/properties-of-the-functioning-of-the-promoter-of-the-microcin-c51-qwmqIzcEk8
Publisher
Springer Journals
Copyright
Copyright © 2001 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/A:1016717430649
Publisher site
See Article on Publisher Site

Abstract

The level of transcription from the promoter of the microcin C51 operon (P mcc ) depends on the growth phase of Escherichia colicells: transcription proceeds with low efficiency at the exponential phase of growth and with higher efficiency when growth of cells is delayed during entry into the stationary phase. The functioning of P mcc was studied in cells grown in different media by a single-copy construct, which contained the cloned promoter region of the microcin C51 operon and the promoterless lacoperon. A decrease in the rate of cell growth caused by changes in the sole carbon source in minimal medium correlated with an increase in the level of transcription from the P mcc promoter at the exponential phase of growth; the expression of P mcc –lacduring cell entry into the stationary phase was higher under less favorable medium conditions. The use of composite rich media impaired this feature. The addition of l-leucine (100 μg/ml) to the medium decreased the expression of P mcc –lacin wild-type cells and those carrying the Δlrpmutation. A further increase in leucine concentration and the presence of other amino acids in the medium enhanced transcription that started from P mcc during cell entry into the stationary growth phase. The capacity of the P mcc promoter and of the wild-type lacZgene promoter upon IPTG induction was virtually the same. A mutation in the ompRgene did not markedly influence transcription started from P mcc .

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 16, 2004

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off