The level of transcription from the promoter of the microcin C51 operon (P mcc ) depends on the growth phase of Escherichia colicells: transcription proceeds with low efficiency at the exponential phase of growth and with higher efficiency when growth of cells is delayed during entry into the stationary phase. The functioning of P mcc was studied in cells grown in different media by a single-copy construct, which contained the cloned promoter region of the microcin C51 operon and the promoterless lacoperon. A decrease in the rate of cell growth caused by changes in the sole carbon source in minimal medium correlated with an increase in the level of transcription from the P mcc promoter at the exponential phase of growth; the expression of P mcc –lacduring cell entry into the stationary phase was higher under less favorable medium conditions. The use of composite rich media impaired this feature. The addition of l-leucine (100 μg/ml) to the medium decreased the expression of P mcc –lacin wild-type cells and those carrying the Δlrpmutation. A further increase in leucine concentration and the presence of other amino acids in the medium enhanced transcription that started from P mcc during cell entry into the stationary growth phase. The capacity of the P mcc promoter and of the wild-type lacZgene promoter upon IPTG induction was virtually the same. A mutation in the ompRgene did not markedly influence transcription started from P mcc .
Russian Journal of Genetics – Springer Journals
Published: Oct 16, 2004
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