Promoter regions of the extA extensin gene from Brassica napus control activation in response to wounding and tensile stress.

Promoter regions of the extA extensin gene from Brassica napus control activation in response to... To identify controlling cis acting promoter regions in the B. napus extA extensin gene, expression in transgenic tobacco of 5′ −159, −433, −664, −789 and −940 bp promoter truncations linked to the uidA (B-glucuronidase) reporter coding sequence were analysed. The −159 and −433 bp truncations directed non specific expression in all cell types within the plant. An activator region which increased expression levels 10 fold in all cell types was located between −159 to −433 bp. A repressor region was found between −664 to −789 bp; removal of this region resulted in a 15 fold increase in expression. Histochemical analysis showed that transgenics containing the −664, −789 and −940 bp truncations directed expression of the fusion gene only in the phloem. A negative regulatory region located between −433 to −664 bp repressed expression in non-phloem cell types. In areas of the plant subject to tensile stress, the repression exerted by the negative regulatory region was overcome, allowing expression in all cell types. The quantitative repressor and activator regions which controlled absolute expression levels in all cell types were seperate from the negative regulatory region which controlled cell type specific expression in response to tensile stress. A wound responsive region was found to be located between −940 to −3500 bp. Thus, the extA gene is under complex control, being regulated by 4 sets of positively and negatively acting cis regions, which control wound inducibility, activation in response to tensile stress, and quantitative expression levels. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Promoter regions of the extA extensin gene from Brassica napus control activation in response to wounding and tensile stress.

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 1998 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1005918816630
Publisher site
See Article on Publisher Site

Abstract

To identify controlling cis acting promoter regions in the B. napus extA extensin gene, expression in transgenic tobacco of 5′ −159, −433, −664, −789 and −940 bp promoter truncations linked to the uidA (B-glucuronidase) reporter coding sequence were analysed. The −159 and −433 bp truncations directed non specific expression in all cell types within the plant. An activator region which increased expression levels 10 fold in all cell types was located between −159 to −433 bp. A repressor region was found between −664 to −789 bp; removal of this region resulted in a 15 fold increase in expression. Histochemical analysis showed that transgenics containing the −664, −789 and −940 bp truncations directed expression of the fusion gene only in the phloem. A negative regulatory region located between −433 to −664 bp repressed expression in non-phloem cell types. In areas of the plant subject to tensile stress, the repression exerted by the negative regulatory region was overcome, allowing expression in all cell types. The quantitative repressor and activator regions which controlled absolute expression levels in all cell types were seperate from the negative regulatory region which controlled cell type specific expression in response to tensile stress. A wound responsive region was found to be located between −940 to −3500 bp. Thus, the extA gene is under complex control, being regulated by 4 sets of positively and negatively acting cis regions, which control wound inducibility, activation in response to tensile stress, and quantitative expression levels.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 6, 2004

References

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