Production of phenolic compounds in callus cultures of tea plant under the effect of 2,4-D and NAA

Production of phenolic compounds in callus cultures of tea plant under the effect of 2,4-D and NAA The effect of hormone-like compounds at different concentrations: 2,4-D (2 × 10−6; 2 × 10−5; and 2 × 10−4M) and 1-NAA (2 × 10−7; 2 × 10−6; 2 × 10−5; 4 × 10−5, and 6 × 10−5 M) on the growth and production of phenolic compounds, including flavans and lignin, was investigated in callus culture of tea plant (Camellia sinensis L., a highly productive strain IFR ChS-2). The growth of the culture was vigorous, and production of phenolic compounds therein was efficient in the medium containing 2 × 10−5 M 2,4-D. Substitution of 1-NAA for 2,4-D in all the cases decelerated the growth of the culture. These changes were more pronounced when 2 × 10−7 and 2 × 10−6 M 1-NAA was used; in this case, biomass accumulation decreased by 1.5–2.0 times as compared with control material growing on the medium with 2 × 10−5 M 2,4-D. In the presence of 1-NAA, the content of total soluble phenolic compounds and flavans in the calli rose by 30% on the average as compared with control material. Accumulation of lignin remained essentially the same. Therefore, the replacement of 2,4-D with 1-NAA in the nutrient medium used for the growing of highly productive strain of tea plant callus did not induce considerable changes in its ability to produce phenolic compounds. Russian Journal of Plant Physiology Springer Journals

Production of phenolic compounds in callus cultures of tea plant under the effect of 2,4-D and NAA

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SP MAIK Nauka/Interperiodica
Copyright © 2008 by MAIK Nauka
Life Sciences; Plant Sciences ; Plant Physiology
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