Production of marker-free plants expressing the gene of the hepatitis B virus surface antigen

Production of marker-free plants expressing the gene of the hepatitis B virus surface antigen The pBM plasmid, carrying the gene of hepatitis B virus surface antigen (HBsAg) and free of any selection markers of antibiotic or herbicide resistance, was constructed for genetic transformation of plants. A method for screening transformed plant seedlings on nonselective media was developed. Enzyme immunoassay was used for selecting transgenic plants with HBsAg gene among the produced regenerants; this method provides for a high sensitivity detection of HBsAg in plant extracts. Tobacco and tomato transgenic lines synthesizing this antigen at a level of 0.01–0.05% of the total soluble protein were obtained. The achieved level of HBsAg synthesis is sufficient for preclinical trials of the produced plants as a new generation safe edible vaccine. The developed method for selecting transformants can be used for producing safe plants free of selection markers. Russian Journal of Genetics Springer Journals

Production of marker-free plants expressing the gene of the hepatitis B virus surface antigen

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SP MAIK Nauka/Interperiodica
Copyright © 2009 by Pleiades Publishing, Ltd.
Biomedicine; Microbial Genetics and Genomics; Animal Genetics and Genomics; Human Genetics
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