Proceedings of the World Molecular Imaging Congress 2015, Honolulu, Hawaii, September 2-5, 2015: General Abstracts

Proceedings of the World Molecular Imaging Congress 2015, Honolulu, Hawaii, September 2-5, 2015:... Mol Imaging Biol (2016) 18 (Suppl 1):S 1Y S1553 DOI: 10.1007/s11307-016-0969-2 Found 978 Records CONTROL ID: 2218021 TITLE: Spectroscopic Photoacoustic Molecular Imaging of Breast Cancer Using an Antibody-Dye Contrast Agent PRESENTER: Katheryne Wilson ABSTRACT BODY: Abstract Body: Introduction: Spectroscopic photoacoustic (sPA) imaging allows for identification of individual optical absorbers within biological tissues, endogenously limited to hemoglobin, melanin, and lipids. Molecularly specific contrast agents are needed to investigate other tissue markers, but few agents are considered readily clinically translatable. Our objective was to explore the potentially clinically translatable combination of antibodies (Ab) and the FDA-approved near-infrared, fluorescent dye indocyanine green (ICG) for sPA imaging of CD276, a molecular marker differentially expressed in breast cancer1, which may improve accuracy of ultrasound imaging for breast cancer detection. Methods: Succinimidyl ester modified ICG was conjugated to monoclonal Ab specific to the CD276 marker. Ab-dye binding ratios were determined using spectrophotometric analysis and protein concentration was determined with a standard BCA assay. A transgenic mouse model for breast cancer development (FVB/N Tg(MMTV/PyMT634Mul) was used to assess the ability of sPA imaging to detect the accumulation of CD276-ICG contrast agent in breast cancer. Mice with invasive breast adenocarcinoma (10-12 weeks of age) were injected intravenously http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Imaging and Biology Springer Journals

Proceedings of the World Molecular Imaging Congress 2015, Honolulu, Hawaii, September 2-5, 2015: General Abstracts

Proceedings of the World Molecular Imaging Congress 2015, Honolulu, Hawaii, September 2-5, 2015: General Abstracts

Mol Imaging Biol (2016) 18 (Suppl 1):S 1Y S1553 DOI: 10.1007/s11307-016-0969-2 Found 978 Records CONTROL ID: 2218021 TITLE: Spectroscopic Photoacoustic Molecular Imaging of Breast Cancer Using an Antibody-Dye Contrast Agent PRESENTER: Katheryne Wilson ABSTRACT BODY: Abstract Body: Introduction: Spectroscopic photoacoustic (sPA) imaging allows for identification of individual optical absorbers within biological tissues, endogenously limited to hemoglobin, melanin, and lipids. Molecularly specific contrast agents are needed to investigate other tissue markers, but few agents are considered readily clinically translatable. Our objective was to explore the potentially clinically translatable combination of antibodies (Ab) and the FDA-approved near-infrared, fluorescent dye indocyanine green (ICG) for sPA imaging of CD276, a molecular marker differentially expressed in breast cancer1, which may improve accuracy of ultrasound imaging for breast cancer detection. Methods: Succinimidyl ester modified ICG was conjugated to monoclonal Ab specific to the CD276 marker. Ab-dye binding ratios were determined using spectrophotometric analysis and protein concentration was determined with a standard BCA assay. A transgenic mouse model for breast cancer development (FVB/N Tg(MMTV/PyMT634Mul) was used to assess the ability of sPA imaging to detect the accumulation of CD276-ICG contrast agent in breast cancer. Mice with invasive breast adenocarcinoma (10-12 weeks of age) were injected intravenously with 33 µg of CD276-ICG or control agents, including isotype Ab conjugated with ICG (Iso-ICG), ICG dye alone, or CD276-ICG in tumor negative mice. Fluorescence, multi-wavelength (680-950 nm, 10 nm increments) sPA, and B-mode ultrasound imaging were performed before and 24h, 48h, and 72h after i.v. administration using the VisualSonics LAZR and the Xenogen IVIS Spectrum. Anatomical B-mode images were used to guide ROI selection for sPA data analysis. Using an in-house sPA data...
Loading next page...
 
/lp/springer_journal/proceedings-of-the-world-molecular-imaging-congress-2015-honolulu-qJQaB9v5uN
Publisher
Springer US
Copyright
Copyright © 2016 by World Molecular Imaging Society
Subject
Medicine & Public Health; Imaging / Radiology
ISSN
1536-1632
eISSN
1860-2002
D.O.I.
10.1007/s11307-016-0969-2
Publisher site
See Article on Publisher Site

Abstract

Mol Imaging Biol (2016) 18 (Suppl 1):S 1Y S1553 DOI: 10.1007/s11307-016-0969-2 Found 978 Records CONTROL ID: 2218021 TITLE: Spectroscopic Photoacoustic Molecular Imaging of Breast Cancer Using an Antibody-Dye Contrast Agent PRESENTER: Katheryne Wilson ABSTRACT BODY: Abstract Body: Introduction: Spectroscopic photoacoustic (sPA) imaging allows for identification of individual optical absorbers within biological tissues, endogenously limited to hemoglobin, melanin, and lipids. Molecularly specific contrast agents are needed to investigate other tissue markers, but few agents are considered readily clinically translatable. Our objective was to explore the potentially clinically translatable combination of antibodies (Ab) and the FDA-approved near-infrared, fluorescent dye indocyanine green (ICG) for sPA imaging of CD276, a molecular marker differentially expressed in breast cancer1, which may improve accuracy of ultrasound imaging for breast cancer detection. Methods: Succinimidyl ester modified ICG was conjugated to monoclonal Ab specific to the CD276 marker. Ab-dye binding ratios were determined using spectrophotometric analysis and protein concentration was determined with a standard BCA assay. A transgenic mouse model for breast cancer development (FVB/N Tg(MMTV/PyMT634Mul) was used to assess the ability of sPA imaging to detect the accumulation of CD276-ICG contrast agent in breast cancer. Mice with invasive breast adenocarcinoma (10-12 weeks of age) were injected intravenously

Journal

Molecular Imaging and BiologySpringer Journals

Published: May 23, 2016

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial