Proceedings of the World Molecular Imaging Congress 2015, Honolulu, Hawaii, September 2-5, 2015: General Abstracts

Proceedings of the World Molecular Imaging Congress 2015, Honolulu, Hawaii, September 2-5, 2015:... Mol Imaging Biol (2016) 18 (Suppl 1):S 1Y S1553 DOI: 10.1007/s11307-016-0969-2 Found 978 Records CONTROL ID: 2218021 TITLE: Spectroscopic Photoacoustic Molecular Imaging of Breast Cancer Using an Antibody-Dye Contrast Agent PRESENTER: Katheryne Wilson ABSTRACT BODY: Abstract Body: Introduction: Spectroscopic photoacoustic (sPA) imaging allows for identification of individual optical absorbers within biological tissues, endogenously limited to hemoglobin, melanin, and lipids. Molecularly specific contrast agents are needed to investigate other tissue markers, but few agents are considered readily clinically translatable. Our objective was to explore the potentially clinically translatable combination of antibodies (Ab) and the FDA-approved near-infrared, fluorescent dye indocyanine green (ICG) for sPA imaging of CD276, a molecular marker differentially expressed in breast cancer1, which may improve accuracy of ultrasound imaging for breast cancer detection. Methods: Succinimidyl ester modified ICG was conjugated to monoclonal Ab specific to the CD276 marker. Ab-dye binding ratios were determined using spectrophotometric analysis and protein concentration was determined with a standard BCA assay. A transgenic mouse model for breast cancer development (FVB/N Tg(MMTV/PyMT634Mul) was used to assess the ability of sPA imaging to detect the accumulation of CD276-ICG contrast agent in breast cancer. Mice with invasive breast adenocarcinoma (10-12 weeks of age) were injected intravenously http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Imaging and Biology Springer Journals

Proceedings of the World Molecular Imaging Congress 2015, Honolulu, Hawaii, September 2-5, 2015: General Abstracts

Proceedings of the World Molecular Imaging Congress 2015, Honolulu, Hawaii, September 2-5, 2015: General Abstracts

Mol Imaging Biol (2016) 18 (Suppl 1):S 1Y S1553 DOI: 10.1007/s11307-016-0969-2 Found 978 Records CONTROL ID: 2218021 TITLE: Spectroscopic Photoacoustic Molecular Imaging of Breast Cancer Using an Antibody-Dye Contrast Agent PRESENTER: Katheryne Wilson ABSTRACT BODY: Abstract Body: Introduction: Spectroscopic photoacoustic (sPA) imaging allows for identification of individual optical absorbers within biological tissues, endogenously limited to hemoglobin, melanin, and lipids. Molecularly specific contrast agents are needed to investigate other tissue markers, but few agents are considered readily clinically translatable. Our objective was to explore the potentially clinically translatable combination of antibodies (Ab) and the FDA-approved near-infrared, fluorescent dye indocyanine green (ICG) for sPA imaging of CD276, a molecular marker differentially expressed in breast cancer1, which may improve accuracy of ultrasound imaging for breast cancer detection. Methods: Succinimidyl ester modified ICG was conjugated to monoclonal Ab specific to the CD276 marker. Ab-dye binding ratios were determined using spectrophotometric analysis and protein concentration was determined with a standard BCA assay. A transgenic mouse model for breast cancer development (FVB/N Tg(MMTV/PyMT634Mul) was used to assess the ability of sPA imaging to detect the accumulation of CD276-ICG contrast agent in breast cancer. Mice with invasive breast adenocarcinoma (10-12 weeks of age) were injected intravenously with 33 µg of CD276-ICG or control agents, including isotype Ab conjugated with ICG (Iso-ICG), ICG dye alone, or CD276-ICG in tumor negative mice. Fluorescence, multi-wavelength (680-950 nm, 10 nm increments) sPA, and B-mode ultrasound imaging were performed before and 24h, 48h, and 72h after i.v. administration using the VisualSonics LAZR and the Xenogen IVIS Spectrum. Anatomical B-mode images were used to guide ROI selection for sPA data analysis. Using an in-house sPA data...
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Publisher
Springer Journals
Copyright
Copyright © 2016 by World Molecular Imaging Society
Subject
Medicine & Public Health; Imaging / Radiology
ISSN
1536-1632
eISSN
1860-2002
D.O.I.
10.1007/s11307-016-0969-2
Publisher site
See Article on Publisher Site

Abstract

Mol Imaging Biol (2016) 18 (Suppl 1):S 1Y S1553 DOI: 10.1007/s11307-016-0969-2 Found 978 Records CONTROL ID: 2218021 TITLE: Spectroscopic Photoacoustic Molecular Imaging of Breast Cancer Using an Antibody-Dye Contrast Agent PRESENTER: Katheryne Wilson ABSTRACT BODY: Abstract Body: Introduction: Spectroscopic photoacoustic (sPA) imaging allows for identification of individual optical absorbers within biological tissues, endogenously limited to hemoglobin, melanin, and lipids. Molecularly specific contrast agents are needed to investigate other tissue markers, but few agents are considered readily clinically translatable. Our objective was to explore the potentially clinically translatable combination of antibodies (Ab) and the FDA-approved near-infrared, fluorescent dye indocyanine green (ICG) for sPA imaging of CD276, a molecular marker differentially expressed in breast cancer1, which may improve accuracy of ultrasound imaging for breast cancer detection. Methods: Succinimidyl ester modified ICG was conjugated to monoclonal Ab specific to the CD276 marker. Ab-dye binding ratios were determined using spectrophotometric analysis and protein concentration was determined with a standard BCA assay. A transgenic mouse model for breast cancer development (FVB/N Tg(MMTV/PyMT634Mul) was used to assess the ability of sPA imaging to detect the accumulation of CD276-ICG contrast agent in breast cancer. Mice with invasive breast adenocarcinoma (10-12 weeks of age) were injected intravenously

Journal

Molecular Imaging and BiologySpringer Journals

Published: May 23, 2016

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