Aqueous dispersions of membrane phospholipids comprised of multilamellar vesicles (MLVs) will spontaneously transform to a stable unilamellar structure equivalent to the membrane bilayer, but only at a critical temperature T*. Since much of the evidence for this transformation derives from equilibrium thermodynamic studies, a description of the molecular and topological events occurring as the critical unilamellar state assembles has not previously been possible. Here we report experiments that provide evidence of a spontaneous topological change from MLVs towards unilamellar vesicles (ULVs) at T*. By applying a shearing stress to vesicle suspensions we have observed a decrease of approximately 25% in the force required to cause bilayers to leak; this decrease is confined to temperatures near T*. The T* values observed agree with those previously obtained by equilibrium methods. Using the method with total lipid extracts from normal biological membranes confirms that T* equals the physiological temperature of the original membranes.
The Journal of Membrane Biology – Springer Journals
Published: Apr 1, 1997
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera